Abstract
Japanese pear (Pyrus pyrifolia) exhibits the S-RNase-based gametophytic self-incompatibility where the pollen-part determinant, pollen S, had long remained elusive. Recent identification of S locus F-box brothers (SFBB) in Japanese pear and apple suggested that the multiple F-box genes are the pollen S candidates as they exhibited pollen specific expression, S haplotype-specific polymorphisms and linkage to the S locus. In Japanese pear, three SFBBs were identified from a single S haplotype, and they were more homologous to other haplotype genes of the same group (i.e., α-, β- and γ-groups). In this study, we isolated new seven PpSFBB −γ genes from different S genotypes of Japanese pear. These genes showed S haplotype-specific polymorphisms, however, sequence similarities among them were very high. Based on the sequence polymorphisms of the PpSFBB −γ genes, we developed a CAPS/dCAPS system for S genotyping of the Japanese pear cultivars. This new S genotyping system was found to not only be able to discriminate the S 1–S 9, but also be suitable for identification of the mutant S 4sm haplotype for the breeding of self-compatible cultivars, and detection of new S haplotypes such as S k.
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Acknowledgments
We thank Ms Yohko Yamamoto, Mr Tatsuya Hirai and Mr Hisao Katsura of Chiba Prefectural Agriculture Research Center for plant materials. We also thank Dr Koichiro Ushijima of Okayama University, Dr Hoy-Taek Kim and Mr Alejandro Raul Puerta of Chiba University for critical reading of the manuscript. This work was supported by Grants-in-Aid for Young Scientists (A, 16688001) from the Ministry of Education, Culture, Sports, Science and Technology of Japan to H.S.
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Communicated by P. Puigdomenech.
An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-008-0619-5
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Kakui, H., Tsuzuki, T., Koba, T. et al. Polymorphism of SFBB −γ and its use for S genotyping in Japanese pear (Pyrus pyrifolia). Plant Cell Rep 26, 1619–1625 (2007). https://doi.org/10.1007/s00299-007-0386-8
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DOI: https://doi.org/10.1007/s00299-007-0386-8