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Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.

  • Cell Biology and Morphogenesis
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Abstract

A novel genic male sterile (GMS) line in Brassica napus L., which was identified in 1999, was found to be controlled by a monogenic dominant gene, which we have designated as MDGMS. The microspores of the MDGMS abort before the degradation of the tapetal cell layer. The F1 fertility from any fertile lines crossed with MDGMS segregated and the ratio was close to 1:1. Bulked segregation analysis (BSA) was employed to identify random amplified polymorphic DNA (RAPD) markers linked to the Ms gene in MDGMS. Among 880 random 10-mer oligonucleotide primers screened against the bulk DNA of sterile and fertile, one primer S243 (5′-CTATGCCGAC-3′) gave a repeatable 1500-bp DNA polymorphic segment S2431500 between the two bulks. Analysis of individual plants of each bulks and other types of GMS and cytoplasmic male sterility (CMS) lines suggest that the RAPD marker S2431500 is closely linked to the MDGMS locus in rapeseed. This RAPD marker has been converted into sequence characterized amplified region (SCAR) marker to aid identification of male-fertility genotypes in segregating progenies of MDGMS in marker-assisted selection (MAS) breeding programs.

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Abbreviations

BSA:

Bulked segregation analysis

CMS:

Cytoplasmic male sterility

GMS:

Genic male sterility

MAS:

Marker assisted selection

MDGMS:

Monogenic dominant GMS

PCR:

Polymerase chain reaction

RAPD:

Random amplified polymorphic DNA

SCAR:

Sequence characterized amplified region

STS:

Sequence tagged site

References

  • Altschul SF, Madden TL, Schäffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  PubMed  CAS  Google Scholar 

  • Barua UM, Chalmers KJ, Hackett CA, Thomas WT, Powell W, Waugh R (1993) Identification of RAPD markers linked to a Rhynchosporium secalis resistance locus in barley using near-isogenic lines and bulked segregant analysis. Heredity 71:177–184

    PubMed  CAS  Google Scholar 

  • Chen FX, Hu BC, Li C, Zhang ML (1995) Success in the breeding of all maintainer for recessive genic male sterile in Brassica napus L. Scientia Agricultura Sinica 28:94–95

    Google Scholar 

  • Chung CT, Niemela SL, Miller RH (1989) One-step preparation of competent E. coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA 86:2172–2175

    Article  PubMed  CAS  Google Scholar 

  • Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349

    Article  PubMed  CAS  Google Scholar 

  • Fu TD (1981) Production and research on rapeseed in the People’s Republic of China. Cruciferae Newslett 6:6–7

    Google Scholar 

  • Giovannoni JJ, Wing RA, Ganal MW, Tanksley SD (1991) Isolation of molecular markers from specific chromosomal intervals using DNA pools from existing mapping populations. Nucleic Acids Res 19:6553–6558

    Article  PubMed  CAS  Google Scholar 

  • Haley SD, Miklas PN, Stavely JR, Byrum J, Kelly JD (1993) Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor Appl Genet 86:505–512

    Article  CAS  Google Scholar 

  • Hormaza JI (1999) Early selection in cherry combining RAPDs with embryo culture. Scien Hort 79:121–126

    Article  Google Scholar 

  • Li DR (1986) Success in and large-scale extention of the breeding of male sterile line, maintenance line and restorer line in rapeseed (Brassica napus L.). Scientia Agricultura Sinica 19:17

    Google Scholar 

  • Mathias R (1985) A new dominant gene for male sterility in rapeseed, Brassica napus L. Z Pflanzenzuchtung 94:170–173

    Google Scholar 

  • McVetty PBE (1997) Cytoplasmic male sterility. In: Shivanna KR and Sawhney VK (eds), Pollen biotechnology for crop production and improvement. Cambridge University Press, pp 155–182

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Miklas PN, Stavely JR, Kelly JD (1993) Identification and potential use of a molecular marker for rust resistance in common bean. Theor Appl Genet 85:745–749

    Article  CAS  Google Scholar 

  • Ogura H (1968) Studies on the new male-sterility in Japanese radish with specific reference to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ 6:39–78

    Google Scholar 

  • Reiter RS, Williams JG, Feldman KA, Rafalsk JA, Tingeyt SV, Scolnik PA (1992) Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs. Proc Nati Acad Sci USA 89:1477–1481

    Article  CAS  Google Scholar 

  • Rives M (1957) Etudes sur la sélection du colza d’hiver. Ann Amelior Plant 1:61–107

    Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (Eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

  • Shiga T, Baba S (1971) Cytoplasmic male sterility in rape plants (Brassica napus L.). Jpn J Breed 21:16–17

    Google Scholar 

  • Snowdon RJ, Friedt W (2004) Molecular markers in Brassica oilseed breeding: current status and future possibilities. Plant Breed 123:1–8

    Article  CAS  Google Scholar 

  • Sorreels ME, Fritz SE (1982) Application of a dominant male-sterility allele to the improvement of self-pollinated crops. Crop Sci. 22:1033–1035

    Article  Google Scholar 

  • Wang H, Wang DJ, Tan XL, Hu XP, Li DR (2001) A simple and rapid method for the preparation of plant genomic DNA by RAPD analysis. Acta Agriculturae Boreal-Occident Sin 10:32–34

    Google Scholar 

  • Williams CE, St Clair DA (1993) Phenetic relationships and levels of variability detected by restriction fragment length polymorphism and random amplified polymorphic DNA analysis of cultivated and wild accessions of lycopersicon esculentum. Genome 36:619–630

    CAS  PubMed  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalsky JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  • Yang GS, Fu TD, Ma CZ, Yang XN (1996) Establishment of a random mating population of polima cytoplasmic male sterility restorers in Brasica napus L. J Huazhong Agric Univ 15:316–321

    Google Scholar 

  • Yang GS, Fu TD (1997) Genetic investigations of three lines with recessive genic male sterility in Brassica napus L. Plant Breed 116:296–298

    Article  Google Scholar 

  • Zhou YM (1993) Recurrent Selection in Brassica napus L. I. Transfer of dominant male sterile gene into genotypes with various types of cytoplasm. Acta Agronomica Sinica 19:70–76

    Google Scholar 

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Acknowledgments

We thank Dr. W.X. Shan of Northwest A&F University and Dr. X.L. Tan of Jiangsu University for the critical reading of the manuscript and their helpful comments. This work was supported by the Chinese National Progress for “863” (No. 2001AA241111) and Nature Science Foundation of Shaanxi Province (No. 2004C108).

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Authors and Affiliations

  1. College of Life Science, Northwest A & F University, Yangling, Shaanxi, 712100, China

    Dao-Jie Wang, Ai-Guang Guo & Fei Huang

  2. Hybrid Rapeseed Research Center of Shaanxi Province, Dali, Shaanxi, 715105, China

    Dao-Jie Wang, Dian-Rong Li & Jian-Hua Tian

  3. Biology Department, Saint Mary’s University, Halifax, Nova Scotia, B3H 3C3, Canada

    Gen-Lou Sun

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  1. Dao-Jie Wang
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  2. Ai-Guang Guo
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  3. Dian-Rong Li
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  4. Jian-Hua Tian
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  5. Fei Huang
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  6. Gen-Lou Sun
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Correspondence to Ai-Guang Guo.

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Communicated by R. J. Rose

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Wang, DJ., Guo, AG., Li, DR. et al. Cytological and molecular characterization of a novel monogenic dominant GMS in Brassica napus L.. Plant Cell Rep 26, 571–579 (2007). https://doi.org/10.1007/s00299-006-0276-5

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