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Influence of IBA and aphidicolin on DNA synthesis and adventitious root regeneration from Malus ‘Jork 9’ stem discs

  • Cell Biology and Morphogenesis
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An Erratum to this article was published on 28 April 2007

Abstract

Adventitious root formation in Malus ‘Jork 9’ stem discs was studied through temporarily blocking DNA synthesis by application of aphidicolin (AD). Higher number of roots per disc (8.4) after 21 days of cultivation were formed after a 24-h pulse of 15 μM AD, compared to control without AD application (6.7), with significantly more roots (3.7) already appearing at day 7, compared to 1.5 roots on the control. The promotive effect of AD on rooting was lower at 5 μM, while a concentration of 30 μM was slightly inhibitory. Results show that DNA synthesis is effectively blocked by AD, and this blockage is overcome after AD withdrawal. The data indicate that AD treatment influences cell divisions, thereby, might synchronise root initiation. The effects of different treatments with and without AD were studied at the cellular level by visualising DNA replication through BrdU-labelling. BrdU labelling further revealed temporal changes in the competence of the explants to respond to applied IBA. Thus, it is shown that the proportion of replicating nuclei present during 28–32 h is significantly increased in the split IBA treatment (0–4 h and 28–32 h; treatment C3), compared with a single IBA application during 0–8 h (treatment C3.1).

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Abbreviations

AD:

Aphidicolin

BAP:

6-Benzylaminopurine

BM:

Basal rooting medium

BrdU:

5-Bromo-2′-deoxyuridine-5′-monophosphate

IBA:

Indole-3-butyric acid

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Acknowledgements

We thank Dr. Frédéric Dubois for technical advice in the immunolocalisation of BrdU-labelled DNA and Jan-Eric Englund for help with statistical analysis.

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Authors and Affiliations

  1. Department of Crop Science, Swedish University of Agricultural Sciences, P.O. Box 44, 23053, Alnarp, Sweden

    Monika Sedira & Margareta Welander

  2. Fachgebiet Botanik, Forschungsanstalt Geisenheim, 65366, Geisenheim, Germany

    Thomas Geier

Authors
  1. Monika Sedira
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  2. Margareta Welander
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  3. Thomas Geier
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Corresponding author

Correspondence to Margareta Welander.

Additional information

Communicated by K. K. Kamo

An erratum to this article can be found at http://dx.doi.org/10.1007/s00299-007-0355-2

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Sedira, M., Welander, M. & Geier, T. Influence of IBA and aphidicolin on DNA synthesis and adventitious root regeneration from Malus ‘Jork 9’ stem discs. Plant Cell Rep 26, 539–545 (2007). https://doi.org/10.1007/s00299-006-0249-8

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