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GISH and AFLP analyses of novel Brassica napus lines derived from one hybrid between B. napus and Orychophragmus violaceus

  • Genetics and Genomics
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Abstract

New Brassica napus inbred lines with different petal colors and with canola quality and increased levels of oleic (∼70%, 10% higher than that of B. napus parent) and linoleic (28%) acids have been developed in the progenies of one B. napus cv. Oro × Orychophragmus violaceus F5 hybrid plant (2n=31). Their genetic constituents were analyzed by using the methods of genomic in situ hybridization (GISH) and amplified fragments length polymorphism (AFLP). No intact chromosomes of O. violaceus origin were detected by GISH in their somatic cells of ovaries and root tips (2n=38) and pollen mother cells (PMCs) with normal chromosome pairing (19 bivalents) and segregation (19:19), though signals of variable sizes and intensities were located mainly at terminal and centromeric parts of some mitotic chromosomes and meiotic bivalents at diakinesis or chromosomes in anaphase I groups and one large patch of chromatin was intensively labeled and separated spatially in some telophase I nuclei and metaphase II PMCs. AFLP analysis revealed that substantial genomic changes have occurred in these lines and O. violaceus–specific bands, deleted bands in ‘Oro’ and novel bands for two parents were detected. The possible mechanisms for these results were discussed.

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Acknowledgements

The study was supported by Hubei Provincial (2002AC015) and National (30070413) Natural Science Foundation of PR China and by a grant from Education Ministry of PR China and by PCSIRT (IRT0442). The critical reading of the manuscript by Prof. Shyam Prakash from Indian Agricultural Research Institute, New Delhi is greatly appreciated

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding Technology, National Center of Oil Crop Improvement (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, P. R. China

    Ni Ma & Zai-Yun Li

  2. Department of Biotechnology, Graduate School of Engineering, Osaka University, Yamada-Oka 2-1, Suita, 565-0871, Japan

    J. A. Cartagena & K. Fukui

Authors
  1. Ni Ma
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  2. Zai-Yun Li
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  3. J. A. Cartagena
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  4. K. Fukui
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Correspondence to Zai-Yun Li.

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Communicated by K. Toriyama

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Ma, N., Li, ZY., Cartagena, J.A. et al. GISH and AFLP analyses of novel Brassica napus lines derived from one hybrid between B. napus and Orychophragmus violaceus . Plant Cell Rep 25, 1089–1093 (2006). https://doi.org/10.1007/s00299-006-0171-0

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  • DOI: https://doi.org/10.1007/s00299-006-0171-0

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