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Expression of endo-1,4-β-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening

  • Genetic Transformation and Hybridization
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Abstract

Arabidopsis thaliana CEL1 protein was detected in young expanding tissues. Immunostaining revealed that CEL1 accumulated mostly in xylem cells. The primary, as well as the secondary xylem showed considerable CEL1 staining. CEL1 was also observed in young epidermal cells, in which the thicker lateral and tangential walls stained more intensely than the inner walls. In newly formed cell walls, the lateral tangential walls were labeled more intensively than the inner walls. Cellulase activity was found to be significantly higher in growing tissue compared to mature parts of the plant. Cel1 expression concurrently with cellulase activity could be restored in detached matured leaves by sucrose treatment after 48 h in the culture medium.

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Authors and Affiliations

  1. CBD-Technologies Ltd., Tamar Science Park, P.O. Box 199, Rehovot, 76100, Israel

    Ziv Shani, Mara Dekel, Miri Horowitz & Natalia Kolosovski

  2. The Robert Smith Institute of Plant Sciences and Genetics, and The Otto Warburg Minerva Center for Agricultural Biotechnology, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot, 76100, Israel

    Mara Dekel, Levava Roiz, Shaul Lapidot, Sari Alkan, Galit Tsabary, Raphael Goren & Oded Shoseyov

  3. Department of Genomics, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel

    Hinanit Koltai

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  1. Ziv Shani
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  2. Mara Dekel
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  3. Levava Roiz
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  4. Miri Horowitz
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  5. Natalia Kolosovski
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  6. Shaul Lapidot
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  7. Sari Alkan
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  8. Hinanit Koltai
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  9. Galit Tsabary
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  10. Raphael Goren
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  11. Oded Shoseyov
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Correspondence to Oded Shoseyov.

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Communicated by L. Jouanin

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Shani, Z., Dekel, M., Roiz, L. et al. Expression of endo-1,4-β-glucanase (cel1) in Arabidopsis thaliana is associated with plant growth, xylem development and cell wall thickening. Plant Cell Rep 25, 1067–1074 (2006). https://doi.org/10.1007/s00299-006-0167-9

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  • DOI: https://doi.org/10.1007/s00299-006-0167-9

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