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Establishment of embryogenic cell suspension cultures of garlic (Allium sativum L.), plant regeneration and biochemical analyses

  • Cell Biology and Morphogenesis
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Abstract

Embryogenic cell suspension cultures of garlic (Allium sativum L.) were initiated in liquid medium from friable embryogenic tissue. The optimal parameters for culture maintenance were: (1) an initial cell density of 1–4% (v/v); (2) medium renewal every 14 days and subculturing every 28 days; (3) a low 2,4-dichlorophenoxyacetic acid concentration (0.1–0.3 mg/l). Cultures regenerated during a 14-month period. The cell suspension cultures differentiated embryos following transfer to a semi-solid embryo induction medium, with histological studies confirming and characterising the embryogenic nature of the process. Forty percent of these embryos converted into plantlets, which produced micro bulbs in vitro. The composition of the sulphur compounds of the micro bulbs obtained from cell suspension embryo-derived plantlets differed slightly from those produced by in vitro shoot proliferation-derived plantlets, but after two cycles of multiplication in the field these differences had disappeared.

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Abbreviations

BAP:

6-Benzylaminopurine

CS:

Cell suspension-derived plantlet

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

NAA:

α-Naphthaleneacetic acid

SCV:

Sedimented cell volume

SP:

In vitro shoot proliferation-derived plantlet

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Acknowledgements

This work was supported by a grant from the EU for the Garlic & Health research program of the 5th PCRDT, Quality of Life (QLK1-1999-00498). We thank the CIRAD-FLHOR Biometrics Department (Xavier Perrier, Cécile Dubois, Jean Pierre Jacquemoud) for the statistical analyses

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Authors and Affiliations

  1. CIRAD-FLHOR, Boulevard de la Lironde, 34398, Montpellier Cedex 5, France

    L. Fereol, S. Causse, D. Triaire & R. Kahane

  2. AVRDC, P.O. Box 42, 741, Shanhua, Tainan, Taiwan, ROC

    L. Fereol

  3. Pathologie Végétale, INRA, BP 94, 84143, Montfavet, France

    V. Chovelon

  4. IRBI, UMR CNRS 6035, Université F. Rabelais, Faculté des Sciences et Techniques, Avenue Monge, 37200, Tours, France

    I. Arnault & J. Auger

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  1. L. Fereol
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  2. V. Chovelon
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  3. S. Causse
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  4. D. Triaire
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  5. I. Arnault
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  6. J. Auger
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  7. R. Kahane
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Correspondence to L. Fereol.

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Communicated by M. Beckert

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Fereol, L., Chovelon, V., Causse, S. et al. Establishment of embryogenic cell suspension cultures of garlic (Allium sativum L.), plant regeneration and biochemical analyses. Plant Cell Rep 24, 319–325 (2005). https://doi.org/10.1007/s00299-005-0937-9

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  • DOI: https://doi.org/10.1007/s00299-005-0937-9

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