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Introduction of the tobacco retrotransposon Tto1 into diploid potato

  • Genetic Transformation and Hybridization
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Abstract

The tobacco retrotransposon Tto1 is one of the few known active retrotransposons, and its transposition in tobacco, rice and Arabidopsis has been reported to be activated by tissue culture. We introduced Tto1 into a diploid potato by means of Agrobacterium-mediated transformation and obtained two transformed plants carrying Tto1. We then induced plant regeneration via callus formation from leaf explants of Tto1-transformed plants and determined the copy number of Tto1 in the regenerants. A drastic increase in the number of Tto1 copies was not observed. Transcripts of Tto1 were detected in the leaf but not in the callus, suggesting that the transposition of Tto1 may not be activated by tissue culture in potato. The results indicated that the potato is a recalcitrant plant with respect to Tto1 transposition and that the behavior of Tto1 can differ depending on the host plant.

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Acknowledgements

We are grateful to Dr. H. Hirochika (National Institute of Agrobiological Sciences) for providing the plasmid and to Dr. M. Mori (Department of Integrated Research for Agriculture) for providing the plant materials. We also thank Dr. K. Saito and Dr. W. Maruyama-Funatsuki (National Agricultural Research Center for Hokkaido Region) for their valuable suggestions and encouragement. This work was supported in part by grant from Japan Society for the Promotion of Science

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  1. National Agricultural Research Center for Hokkaido Region, Sapporo, 062-8555, Japan

    Takuma Ishizaki & Akira Kato

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  1. Takuma Ishizaki
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  2. Akira Kato
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Correspondence to Takuma Ishizaki.

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Communicated by H. Ebinuma

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Ishizaki, T., Kato, A. Introduction of the tobacco retrotransposon Tto1 into diploid potato. Plant Cell Rep 24, 52–58 (2005). https://doi.org/10.1007/s00299-005-0919-y

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