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Organogenesis and plant regeneration in Taxus wallichiana (Zucc.)

  • Cell Biology and Morphogenesis
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Abstract

We describe an efficient process for regeneration of Taxus wallichiana plants via shoot organogenesis from callus cultures derived from zygotic embryos. Zygotic embryos cultured on half strength Lloyd and McCown's basal medium supplemented with SH vitamin (½ WPMSH), 0.5 mg l−1 6-benzyladenine (BA) in combination with 1.0–2.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) or α-Napthaleneacetic acid (NAA) produced two morphologically distinct types of calli-compact, green callus (CG) and compact, yellow (CY) callus after 4 weeks of culture. Optimum frequency (63%) of adventitious shoot bud induction was achieved in CG callus (3.0±0.67 shoot buds per gram of CG callus) when cultured on ½ WPMSH basal medium supplemented with 2.5 mg l−1 BA after 4 weeks. The inclusion of 1% activated charcoal (AC) to ½ WPMSH basal medium (shoot elongation medium) led to maximum shoot elongation (2.15 cms). Microshoots rooted in high frequency (40%) in MS basal medium in which the concentration of nitrates was reduced to one-fifth the normal concentration after 4 months of culture.

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Abbreviations

BA:

6-Benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

NAA:

α-Napthaleneacetic acid

AC:

Activated charcoal

CG:

Compact, green callus

CY:

Compact, yellow callus

FC:

Friable, creamish callus

IBA:

Indole-3-butyric acid

IAA:

Indole-3-acetic acid

Kn:

Kinetin

2iP:

2-Isopentyladenine

TDZ:

Thidiazuron

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Acknowledgement

Financial assistance from Indo-French Centre for the Promotion of Advanced Research (Centre Franco-Indien pour la Promotion de la Recherche Avancée) is gratefully acknowledged (Project 2503-2).

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Authors and Affiliations

  1. Centre of Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India

    Mukul Manjari Datta, Anrini Majumder & Sumita Jha

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  1. Mukul Manjari Datta
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  2. Anrini Majumder
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  3. Sumita Jha
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Correspondence to Sumita Jha.

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Communicated by G.C. Phillips

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Datta, M.M., Majumder, A. & Jha, S. Organogenesis and plant regeneration in Taxus wallichiana (Zucc.). Plant Cell Rep 25, 11–18 (2006). https://doi.org/10.1007/s00299-005-0027-z

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