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Chalcone synthase-like gene in the liverwort, Marchantia paleacea var. diptera

  • Genetics and Genomics
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Abstract

A chalcone synthase (CHS)-like gene, MpCHSLK1, was isolated from liverwort, Marchantia paleacea var. diptera. Phylogenetic analysis revealed that MpCHSLK1 is closely related to stilbene synthase of the whisk fern, Psilotum nudum. Southern blot analysis using an MpCHSLK1 probe revealed that the gene belongs to a small gene family. Northern blot analysis indicated that CHS-like genes were expressed in either the mother plants or photoautotrophic cells. In photoautotrophic cells, the CHS-like genes were expressed light-dependently, and this expression was completely inhibited by the photosynthetic electron transport inhibitor, DCMU.

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Abbreviations

CHS:

Chalcone synthase

DCMU:

3-(3′,4′-Dichlorophenyl)-1-1-dimethylurea

POR :

Protochlorophyllide oxidoreductase

STS:

Stilbene synthase

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Acknowledgements

This work was supported by a grant from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN; to H.T.).

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Authors and Affiliations

  1. Yatsushiro National College of Technology, Hirayama-Shinmachi, Yatsushiro, Kumamoto, 866-8501, Japan

    Shuichi Harashima

  2. Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1, Kumamoto, 860-8555, Japan

    Hiroyoshi Takano

  3. Department of Biological Science, Faculty of Science, Kumamoto University, Kurokami 2-39-1, Kumamoto, 860-8555, Japan

    Kanji Ono

  4. Center for Marine Environment Studies, Kumamoto University, Kurokami 2-39-1, Kumamoto, 860-8555, Japan

    Susumu Takio

Authors
  1. Shuichi Harashima
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  2. Hiroyoshi Takano
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  3. Kanji Ono
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  4. Susumu Takio
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Corresponding author

Correspondence to Susumu Takio.

Additional information

Communicated by I.S. Chung

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Harashima, S., Takano, H., Ono, K. et al. Chalcone synthase-like gene in the liverwort, Marchantia paleacea var. diptera . Plant Cell Rep 23, 167–173 (2004). https://doi.org/10.1007/s00299-004-0808-9

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  • DOI: https://doi.org/10.1007/s00299-004-0808-9

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