Abstract
Explants of mature vigorous donor trees of wych elm (Ulmus glabra Huds.) that had not been previously exposed to Dutch elm disease were investigated for the influence of phytohormones and media on shoot multiplication rates and organogenic capacity. The regenerates were micropropagated from cultures that originated from 15-year-old progeny of plus trees. Two plus trees aged over 70 years showed recalcitrant responses. Thidiazuron in combination with 6-benzylaminopurine (BAP) induced a significantly higher number of shoots per explant than the most optimal BAP treatment (5.88 vs. 3.05 shoots). Woody plant medium and Dubovský minimal medium had no significant effects on shoot formation and multiplication rates. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions. Two experimental field plots with 3-year-old in vitro-propagated trees were established.
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Abbreviations
- DED::
-
Dutch elm disease
- BAP::
-
6-Benzylaminopurine
- IBA::
-
Indole-3-butyric acid
- TDZ::
-
Thidiazuron
- WPM::
-
Woody Plant Medium
- DM::
-
Dubovský Minimal Medium
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Acknowledgements
The authors thank Dr D. Gömöry for the statistical advice. Excellent technical and laboratory assistance by Ľ. Lohinská, J. Lelková, H. Vaňová and M. Mišenko is greatly acknowledged. This work was financed by the Slovak Grant Agency VEGA 1/0201/03 and 1/0635/03, and by Lesy S.R., a state-owned company.
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Biroščíková, M., Spišáková, K., Lipták, Š. et al. Micropropagation of mature wych elm (Ulmus glabra Huds.). Plant Cell Rep 22, 640–644 (2004). https://doi.org/10.1007/s00299-003-0749-8
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DOI: https://doi.org/10.1007/s00299-003-0749-8