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Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.

  • Genetic Transformation and Hybridization
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Abstract

Somatic embryogenesis from leaf explants of Scaevola aemula R. Br. was achieved. Somatic embryos were induced from explants cultured on MS medium supplemented with 0.2 mg/ 2,4-dichlorophenoxyacetic acid and 0.2–0.5 mg/l 6-benzylaminopurine (BAP). Various developmental stages of somatic embryos were found on this medium—from globular embryos to germinated embryos. The transfer of globular embryos to MS medium containing 0.5 mg/l BAP resulted in a high frequency of shoot regeneration. Leaf explants cultured on MS medium containing different combinations of BAP and α-naphthaleneacetic acid formed adventitious shoots and roots. Histological examination confirmed the process of somatic embryogenesis. Induction of somatic embryogenesis in Scaevola provides a system for studying embryogenesis in Australian native plants and will facilitate the improvement of these plants using genetic transformation techniques.

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Fig. 1A–H
Fig. 2A, B
Fig. 3A—D

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Abbreviations

ABA :

Abscisic acid

BAP :

6-Benzylaminopurine

2,4-D :

2,4-Dichlorophenoxyacetic acid

NAA :

α-Naphthaleneacetic acid

PIPES :

Piperazine-N, N-(2-ethanesulfonic acid)

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Authors and Affiliations

  1. Plant Molecular Biology and Biotechnology Laboratory, Australian Research Council Centre of Excellence for Integrative Legume Research, Institute of Land and Food Resources, The University of Melbourne, Parkville, Victoria, 3010, Australia

    Y-H. Wang & P. L. Bhalla

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  1. Y-H. Wang
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  2. P. L. Bhalla
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Correspondence to P. L. Bhalla.

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Communicated by R.J. Rose

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Wang, YH., Bhalla, P.L. Somatic embryogenesis from leaf explants of Australian fan flower, Scaevola aemula R. Br.. Plant Cell Rep 22, 408–414 (2004). https://doi.org/10.1007/s00299-003-0707-5

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  • DOI: https://doi.org/10.1007/s00299-003-0707-5

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