Abstract
Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo (Bambusa edulis) inflorescences were amplified by incubation in Murashige and Skoog medium supplemented with 0.1 mg/l thidiazuron. Mutant albino inflorescences also proliferated in this medium. This method is equally effective with dicotyledonous plants. Ginseng (Panax ginseng) buds were incubated in B5 medium supplemented with 1 mg/l benzyladenine and 1 mg/l gibberellic acid; new inflorescences developed from the base of the explants. Ginseng flowers were parthenocarpic and some of the fruit proliferated in vitro. Using the inflorescences as the material of somatic embryogenesis, we demonstrated that these were not mutations. The regenerated plants still had a juvenile phase and grew normally.
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Abbreviations
- BA :
-
Benzyladenine
- 2,4-D :
-
2,4-Dichlorophenoxyacetic acid
- GA 3 :
-
Gibberellic acid
- MS :
-
Murashige and Skoog basal medium
- NAA :
-
Naphthaleneacetic acid
- TDZ :
-
Thidiazuron
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Acknowledgements
We thank Ms. Chen-Chen Shih and Mr. Duncan Herbert for helpful discussions during manuscript preparation. This work was supported by grants from Academia Sinica and the National Science Council of Taiwan.
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Communicated by F. Sato
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Lin, CS., Chen, CT., Lin, CC. et al. A method for inflorescence proliferation. Plant Cell Rep 21, 838–843 (2003). https://doi.org/10.1007/s00299-003-0571-3
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DOI: https://doi.org/10.1007/s00299-003-0571-3