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Roles of putative His-to-Asp signaling modules HPT-1 and RRG-2, on viability and sensitivity to osmotic and oxidative stresses in Neurospora crassa

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Abstract

Neurospora crassa has a putative histidine phosphotransfer protein (HPT-1) that transfers signals from 11 histidine kinases to two putative response regulators (RRG-1 and RRG-2) in its histidine-to-aspartate phosphorelay system. The hpt-1 gene was successfully disrupted in the os-2 (MAP kinase gene) mutant, but not in the wild-type strain in this study. Crossing the resultant hpt-1; os-2 mutants with the wild-type or os-1 (histidine kinase gene) mutant strains produced no progeny with hpt-1 or os-1; hpt-1 mutation, strongly suggesting that hpt-1 is essential for growth unless downstream OS-2 is inactivated. hpt-1 mutation partially recovered the osmotic sensitivity of os-2 mutants, implying the involvement of yeast Skn7-like RRG-2 in osmoregulation. However, the rrg-2 disruption did not change the osmotic sensitivity of the wild-type strain and the os-2 mutant, suggesting that rrg-2 did not participate in the osmoregulation. Both rrg-2 and os-2 single mutation slightly increased sensitivity to t-butyl hydroperoxide, and rrg-2 and hpt-1 mutations increased the os-2 mutant’s sensitivity. Although OS-1 is considered as a positive regulator of OS-2 MAP kinase, our results suggested that HPT-1 negatively regulated downstream MAP kinase cascade, and that OS-2 and RRG-2 probably participate independently in the oxidative stress response in N. crassa.

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Acknowledgments

This work was supported by a research grant from The INOUE ENRYO Memorial Foundation for promoting sciences from Toyo University.

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  1. Isamu Yamaguchi

    Present address: Presidential Office, Agricultural Chemicals Inspection Station (ACIS), 2-772 Suzuki-cho, Kodaira, Tokyo, 187-0011, Japan

Authors and Affiliations

  1. Faculty of Life Sciences, Toyo University, Itakura, Oura-gun, Gunma, 374-0193, Japan

    Shinpei Banno, Rieko Noguchi, Kazuhiro Yamashita, Fumiyasu Fukumori & Makoto Fujimura

  2. Environmental Molecular Biology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Makoto Kimura

  3. Plant Science Center, RIKEN, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa, 230-0045, Japan

    Makoto Kimura & Isamu Yamaguchi

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  1. Shinpei Banno
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Correspondence to Makoto Fujimura.

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Communicated by J. Heitman.

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Banno, S., Noguchi, R., Yamashita, K. et al. Roles of putative His-to-Asp signaling modules HPT-1 and RRG-2, on viability and sensitivity to osmotic and oxidative stresses in Neurospora crassa . Curr Genet 51, 197–208 (2007). https://doi.org/10.1007/s00294-006-0116-8

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  • DOI: https://doi.org/10.1007/s00294-006-0116-8

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