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An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast

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Abstract

Three groups of fungicides (phenylpyrroles, dicarboximides, aromatic hydrocarbons) are effective against filamentous fungi. The target of these fungicides is the osmotic stress signal transduction pathway, which is dependent on the Os-1 family of two-component histidine kinases. These fungicides usually have no fungicidal effect on the yeast Saccharomyces cerevisiae. In this report, we found that expression of Hik1, an Os-1 orthologue from rice blast fungus, can confer fungicide-sensitivity to yeast. This requires both the histidine kinase and the response regulator domains of Hik1. Analysis of yeast mutants indicated that this sensitivity is Hog1- and Ssk1-dependent. In addition, our studies revealed an interaction between Hik1 and Ypd1. These observations suggest that Hik1 is a direct target of the fungicides or is a mediator of fungicide action and that the fungicidal effect is transmitted to the Hog1 pathway via Ypd1.

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Acknowledgements

This study was supported by grants for the Bio-architect and the Ecomolecular Science Research Program of RIKEN. This work was also supported by MEXT.KAKENHI (15780063).

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Authors and Affiliations

  1. RIKEN (Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Takayuki Motoyama, Tomohiro Ohira, Kaori Kadokura, Isamu Yamaguchi & Toshiaki Kudo

  2. Faculty of Life Science, University of Toyo, Itakura, Oura-gun, Gunma, 374-0193, Japan

    Akihiko Ichiishi & Makoto Fujimura

Authors
  1. Takayuki Motoyama
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  2. Tomohiro Ohira
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  3. Kaori Kadokura
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  4. Akihiko Ichiishi
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  5. Makoto Fujimura
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  6. Isamu Yamaguchi
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  7. Toshiaki Kudo
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Correspondence to Takayuki Motoyama.

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Communicated by S. Hohmann

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Motoyama, T., Ohira, T., Kadokura, K. et al. An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast. Curr Genet 47, 298–306 (2005). https://doi.org/10.1007/s00294-005-0572-6

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  • DOI: https://doi.org/10.1007/s00294-005-0572-6

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