Abstract
The synthesized heterocyclic compound: 5-[4-bromobenzylidene amino)-1, 3, 4-thiadiazole-2-thiol abbreviated as BATT possessed good antibacterial activity for various Gram-negative and Gram-positive bacteria. In addition to potent antitumor activity to breast cancer, for the first time, the novelty of this study is facile low-cost formulation of safe antitumor drug delivery system (DDS) for breast cancer from such simple heterocyclic compound. Heterocyclic compound is efficiently and spontaneously incorporated into MMT clay polymer matrix forming novel therapeutic nanocomposite DDS for breast cancer. BATT successfully intercalates MMT clay polymer matrix. Electron donation ability of nanocomposites is confirmed by cyclic voltammetry in terms of small peak-to-peak redox potential \(\Delta E_{{{\text{peak}}}}\). Adsorption of BATT on clay is carried out by batch adsorption method. Better adsorption strength at low pH 2 decreased with increasing pH. Adsorption data are analyzed by multiple mathematical models. MMT clay is an efficient carrier polymer heterogonous adsorbent for BATT at optimum conditions: contact time 2.0 h, pH 2 and initial concentration up to 1200 ppm. Adsorption rate constant, k2 equals 14.6 \(\times\) 10–3 and qe 19 mg g−1 (R2 0.999). pH of zero charge of MMT at pH 3 reflected adsorption mechanism of MMT. At pH 2, BATT is loaded on MMT by physi- and chemisorption. At pH 4, BATT is loaded on MMT by chemisorption.
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Introduction
Nanocomposites drug delivery system (DDS) targeting therapeutic drug for treating cancer is a promising medicinal nanotechnology [1]. In current era of molecular medicine, much efforts have developed therapeutic DDS for breast cancer as nanocomposites of biomaterials [2]. Breast cancer, BC, represents quarter percent of cancer types. It is the second common cancer type that has a high incidence rate. Two million new cases of BC appear annually. It is among the main causes of women death. Most BC begins in breast tissue of glands lobules for milk production, or in ducts connecting lobules to nipple. Remainder breast is made up of fatty, connective and lymphatic tissues [3].
BC is caused by unhealthy lifestyles, long time fertility, using preventive pregnancy hormones and hormone replacement therapy, no children and breastfeeding, obesity after menopause, physical inactivity, alcohol consumption, family history, genetic predisposition and inherited mutations [3]. BC is treated by radiation therapy, targeted chemotherapy and endocrine hormone therapy. Chemotherapy (CT) harms human health by therapeutic antitumor drugs due to poor solubility in aqueous biological fluids, rapid deactivation, low apparent permeability and non-biodegradability [4]. Limitation of CT for BC is insolubility of therapeutic drugs in aqueous media [1]. DDS reduces drug side effects and maximizing drug action. DDS design is the most important nanotechnology. Therapeutic drug should possess high electron density for efficient loading on biocompatible polymer carrier [1]. MMT clay is a hydrated aluminosilicate inorganic polymers, mixed fine-grained clay minerals and metal oxides. MMT is safe drug carrier due to large specific internal surface area, physicochemical stability and several other structural and surface properties. MMT has high adsorption capacity, enhanced rheology, intercalate and reserve large neutral water-insoluble drug molecules into interlayer space 2D aluminosilicate layers. MMT releases incorporated loaded drugs in biological fluids via ion exchange mechanism or adsorption on porous active sites. MMT has high cation exchange capacity CEC for Na+ or K+ ions 1.0 mg equiv g−1. 0.96 nm and 0.94 nm platelet thickness [1]. Mechanism of DDS formulation is mainly adsorption of therapeutic drug onto pores of MMT [5]. MMT is bioactive, biodegradable, hemocompatible and non-toxic [1]. Main objectives of study are preparation and characterization of new nanocomposites of biologically active 5-[4-bromobenzylidene amino)-1, 3, 4-thiadiazole-2-thiol (BATT) compound on bioactive MMT polymer. Compound BATT is selected for adsorption on clay inorganic polymer as it is biologically active compound for simulation new antibacterial and anticancer DDS.
Materials and methods
All chemicals are of analytical grades obtained from Sigma-Aldrich used as received without further purification. BATT is prepared as follows [6]. 1.33 g (0.01 mol) solution: 5-amino-2-mercapto-1,3,4- thiadiazole, AMT, is dissolved in 25 mL absolute ethanol, treated with (1.45 g, 0.01 mol) p-bromobenzaldehyde solution dissolved in 25 mL ethanol. Three drops of glacial acetic acid is added dropwise. Mixture is heated under reflux for 6 h. Solid separated out upon cooling is filtered, dried and crystallized from EtOH/DMF as pale yellow crystals, 87% yield; m.p. 223 °C.
Chemical structure of BATT is confirmed using FTIR absorption vibrational bands (KBr): 2925(SH-); 1610(C=N); 1552 (C=C Ar–H); 1069 (C–N); 1036 (C–H); 825 (C–C–C bending in plane); 712( C-S); 667.20 (C–Br); 539 (C–C–C bending out of plane). 1H NMR (400 M Hz, DMSO-d6) at δH (ppm): 13.0 (1 H singlet, SH group; 9.98 (1 H singlet, –CH=N); 7.80–7.07 (4 H multiple, aromatic protons); 13C NMR (100 MHz, DMSO-d6) δC 181.4; 162.1; 159.2; 132.8; 132.2; 131.8; 129.3; Anal. Cald:C9H6BrN3S2 (300.187 g mol−1): C, 36.01; H, 2.02; N, 13.99; S, 21.36. Found: C, 36.00; H, 2.01; N, 13.89; S, 21.34.
It contains 5-membered heterocyclic thiadiazole nucleus, thiol group and Schiff base synergistically enhanced biological activity. BATT is prepared according to Scheme 1 via reflux p-bromobenzaldehyde with AMT in presence of acetic acid catalyst.
Formation mechanism is illustrated in Scheme 2. p-bromobenzaldehyde is protonated by acetic acid, attacking amino group of thiadiazole nucleus to carbonyl carbon affording iminol which consequently dehydrated by reflux to afford corresponding Schiff`s base BATT.
BAAT and nanocomposites are characterized using: Fourier transform infrared (FTIR) spectroscopy, KBr disc, frequency range: 200–4000 cm−1 using Bruker TENSOR 37 spectrophotometer, model 1430 calibrated by polystyrene film (1602 ± 1 cm−1), nuclear magnetic resonance 1H NMR and 13C NMR, Bruker spectrometer (400 MHz). Chemical shift (δ ppm) is relative to TMS internal standard. m.p. is determined with a Mel-Temp. apparatus and uncorrected. TLC is performed on Baker-Flex silica gel 1B-F plates using ethyl acetate-petroleum ether eluent (1:1v/v); elemental microanalysis; powder X-ray diffraction, pXRD using X-ray Shimadzu XRD-6100 diffractometer; thermogravimetric analysis TGA, differential thermogravimetric; and differential thermal analysis DTA using Shimadz DTG 60H thermal analyzer in platinum crucible at temperature range 20–700 °C under nitrogen and heating rate 10 °C/min. [7, 8].
In vitro antibacterial activity tests at 30 °C using diffusion agar method, well 6.0 mm diameter: 100 µL BATT and ciprofloxacin standard antibiotic. Tested strains are obtained from culture collection Regional Center for Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt). Tested Gram-positive, G+ (Staphylococcus aureus, E. coli) and Gram-negative bacteria, G− (Pseudomonas aeruginosa, Bacillus cereus) are cultured on nutrient agar. Antibacterial activity was determined after one day by cut-plug method [8].
Some parameters of optimized structure are calculated by B3LYP/6 method, basis set 31-G d, p level using GAUSSIAN 9 package software, frontier molecular orbitals, HOMO and LUMO are visualized with Gaussian view [10]. Some chemical descriptors are calculated [7]:
Redox characteristic of BATT is clarified via cyclic voltammetry from 0.5 V to − 0.5 V, scan rate 20 mV s−1 using silver–silver chloride reference electrode. Sample is loaded on glassy carbon electrode 0.032 cm2 surface area [9].
BATT is uploaded on clay polymer by adsorption to formulate antibacterial anticancer DDS. In batch adsorption method, adsorption parameters contact time, pH and initial concentration are optimized. For determination maximum adsorption capacity, 100 mg MMT is dispersed in 20 mL de-ionized water contains 15 mg BATT, agitated at room temperature for different contact time (t) up to 3.5 h. Mixture is centrifuged and filtered using poly tetra-fluoro-ethylene (PTFE) filter, 0.25 µm pore size. Residual concentration in supernatant is determined by UV–Vis spectroscopy at λmax. 320 nm [10]. Each adsorption run is carried out in triplicate (n = 3). Average value is calculated along with standard deviation.
where qt is adsorbed quantity mg g−1, Co is initial concentration mg L−1, Ce is equilibrium concentration, mg L−1, m is mass adsorbent, V is solution volume, L.
To optimize pH, 100 mg MMT is treated with 20 mL 15 mg BATT solution at pH range 2–12. pH is adjusted using aqueous solutions 0.1 M NaOH or 0.1 M HCl. To optimize initial concentration, 100 mg MMT is mixed with 10 mL BATT solution containing different ppm concentration BATT.
All adsorption data are carried out in triplicate (n = 3) for calculation qt and % adsorption. All data are represented as mean \(\overline{ x}\) and S.D
In regression analysis for any two variables X, Y
where n is the number of data points, \({Y}_{i},\)…, \({\widehat{Y}}_{i}\) are observed and predicted values, respectively. MSE is calculated from a set X and Y by finding regression line, insert X values into linear regression equation to find \({\widehat{Y}}_{i}\).
To find the best thermodynamic or kinetic model for adsorption data, an error analysis is performed. Sum of squares errors (SSE) and chi-square (\({{\varvec{\chi}}}^{2}\)) are achieved by equations:
where qe exp and qe cal are experimental and predicted adsorption capacities at equilibrium, respectively.
Nanocomposite NC1 is prepared [1]: An aqueous MMT dispersion (1% w/v) is prepared by dispersing 2.0 g MMT in 200 mL double distilled water. Turbid dispersion is left at room temperature for eighteen hrs. for complete swelling. At pH 4.0 of dispersion, 0.2778 g BATT equivalent to excess three CEC of MMT is dissolved in 50 mL DMF and added to MMT dispersion. Mixture is stirred for 4.0 h at 40 °C, left for settling for 18 h at room temperature then filtered. Precipitate washed several times with DMF to remove unbound BATT molecules, dried at room temperature, grinded with mortar and pestle to be fine powder [13]. NC2 is prepared by the same method of NC1 at pH 2.
Colloidal stability of NC1 is confirmed using NanoZS/ZEN3600 Zetasizer (Malvern, Instruments Ltd, Malvern, UK) to determine zeta potential [11].
Results and discussion
All spectral analysis in Fig. 1a–c confirmed chemical structure of BATT . IR spectrum showed characteristic vibrational bands at wavenumber, cm−1: 2925 (SH-); 1610 (C=N); 1552 (C=C Ar–H); 1069 (C–N); 1036 (C–H); 825 (C–C–C bending in plane); 712 (C–S); 667.20 (C–Br); 539 (C–C–C bending out of plan) [1].
1H NMR spectrum (400 MHz, DMSO-d6) showed a singlet at δ 13.0 ppm (SH group; a singlet at 9.98(–CH=N); aromatic protons multiplet at 7.80–7.07 ppm. 13C (DMSO-100 MHz); δ: 181.4; 162.1; 159.2; 132.8; 132.2; 131.8; 129.3. Protons and carbons of BATT molecules are shown in their appropriate positions [12].
BAAT showed potent antibacterial to G− bacteria than ciprofloxacin and moderate activity to G+ bacteria, Table 1.
Antibacterial effect of BATT is more pronounced against G− bacteria (Fig. 2) than G+ bacteria that possess thick cell wall contains massive peptidoglycan. G− bacteria have two layers cell membranes, and outer membrane comprises lipopolysaccharide protective against antibiotics and antibacterial drugs and detergents that could damage inner membrane or cell wall peptidoglycan [8]. IZ for BATT exceeds that of Ciprofloxacin for G− bacteria.
SRB assay in-vitro antitumor activity of BATT is evaluated by monitoring cytotoxicity via comparison between bioavailability McF-5 normal human lung fibroblast and cancer cells lines: McF-7 breast, CACO intestinal colon and liver HepG-2 [1]. Cell lines are sub cultivated, for 120 passages plated in 96-multiwell plate 104 cells/well along one day for fixation on plate walls before adding 0, 1, 2.5, 5 and 10 μg/mL BATT. Each dose is added to cell monolayer triplicates wells, incubated for two days at 37 °C under 5% CO2 atmosphere. Cells are fixed, washed and stained with sulfo-rhodamine-B stain. Excess stain is washed with acetic acid and attached stain is recovered by Tris EDTA buffer. Color optical density (O.D.) is measured in Mindray MR-96A ELISA reader at 570 nm. Plot surviving fraction-concentration gave survival curve of each treated tumor cell. IC50 percent control of infected and uninfected response values is calculated for BATT and Doxorubicin positive standard. Method validation is tested by taking fourfold concentration of blank and control.
Table 2 shows mean \(\overline{x }\) and standard error \(\overline{\mathrm{SE} }\) or S.D. triplicates O.D, for each test solution in mean following Poisson distribution [13]. % viability of tumor and normal cells are determined in triplicates for each concentration, Table 2.
Table 3 collected mean values \(\overline{x }\) and \(\overline{\mathrm{SE} }\) of %cell viability at different concentrations.
Small error \(\overline{\mathrm{SE} }\) in mean value \(\overline{x }\) of tested concentrations confirmed validity and precision of SRB assay method.
Figure 3 shows BATT cytotoxicity to McF-7 cells and McF-5.
Error bars in histogram of cell viability versus concentration (Fig. 3) are due to small S.D. in triplicate data and confirmed high accuracy and precision of SRB assay.
BAAT inhibited availability of McF-7 by 14% at 31.25 mg/L and 15.6 mg/L with low cytotoxicity on normal cells McF-5 [13, 14]. It acts by heterocyclic rings and thiol group. It is recommended to formulation of DDS for BC using MMT safe adsorbent carrier. BATT IC50 at 11 μg/mL is a comparable value to that of Doxorubicin, 8 μg/mL [16].
Efficient BATT loading on MMT matrix is confirmed from FTIR vibrational bands at characteristic frequency, cm−1 (Fig. 4). MMT: 3445.82 (OH); 1637.55 (Si=O); 1049.97 (C-O stretching); 798.28 (Si–Si); 526.24 (Si–O); 468.32 (Al-O).
NC1: 3441.22 (N–H); 1659.63 (Si=O); 1496.35 (C=S); 1439.03 (C-N); 1390.46 (CH=);1048.15 (C-H); 795.13 (C–C–C bending in plane); 667.20, (C–Br); 524.63 (C–C–C bending out of plane); 467.52 (Al-N) cm−1. New bands at 1659.63 (Si=O); 1496.35 (C=S); 1439.03 (C-N); 1390.46 (CH=); 667.20 (C–Br) or (Al–N) confirmed BATT adsorption on MMT. Bands at 3253.09, 3132.60, and 2925.27 symmetric stretching vibration of NH disappeared in NC2 [1].
Figure 5 shows optimized 3D geometrical structure of BATT. Computations are converged upon true minimum energy − 1120 eV ensuring stable ground state. High dipole moment 17.38 Debye reflected polarity charge separation and dielectric character of BAAT electron donner Lewis base.
Figure 6 shows wave functions \(\psi\) of electron density in molecular orbitals of BATT molecule in duplicate due to trans and cis isomers. \(\psi\)HOMO lies on thiadiazole ring and \(\psi\)LUMO on phenyl ring containing vacant p-orbitals [7].
Geometrical parameters atomic Mulliken charge reflecting electron density on atoms, bond lengths, bond angles and dihedral angles confirming molecular structure of BATT and dihedral angle are collected in, supplementary information SI, Table SI.1, 2. [1]. BATT is electrically neutral, stoichiometric formula C9H6N3S2 Br. N13 has highest charge and it is electron donating site. C-H bond length is 1.1 Å. C–C bonds in phenyl group have same bond length 1.4 Å. C–N bond length, 1.3 Å is shorter than C–C bond due to electronegative N atom. Bond angles 120° confirmed trigonal planar sp2 hybridization. Dihedral angles ± 179° are for distorted linear sp2 hybridization. Bond angles less than 90° reflect distorted tetrahedral sp3 hybridization. Dihedral angles ± 180° are due to SP hybridization and molecular planarity. Dihedral angles 180°-0° suggested anti- and syn positions, respectively. Dipole moment on Cartesian coordinates is µx 3.7161, µy − 2.7634, µz − 0.4974. Electron transfer occurs from HOMO BATT into LUMO MMT [1, 7] (Fig. 7).
States per energy interval at each energy level available for electrons occupation density of states, DOS, and Fermi levels are viewed using Gauss Sum 2.2.5 program. Intense green colored DOS reflects many states available for electrons occupation and intense electron density [15] (Fig. 8).
Some molecular chemical activity indices are calculated based on EHOMO and ELUMO. Chemical potential, Pi; electronegativity, χ; global hardness, η; softness, σ and global electrophilicity index, ω, are collected in Table 4. Electron donation ability of BATT to an appropriate acceptor sites is confirmed as η nearly equals ΔELUMO-HOMO (band gap Eg) indicating charge transfer from BATT molecule. Softness confirmed polarizability and inversely proportional to η. Parameters EHOMO, ELUMO and ∆ELUMO-HOMO reflected electron donation ability of BAAT [7].
Small Eg 0.1408 eV showed BATT is polarizable soft Lewis base. Adsorption center SH group chelates MMT clay via coordinate bond formation reinforced by back donation to vacant p-orbital in phenyl group. High EHOMO indicated low ionization potential and high electron donation tendency to Al atoms in MMT with low energy vacant p-orbital in MMT. Low ELUMO indicated stronger electron accepting ability by back donation. Electrophilicity ω measures stabilization by additional electronic charge [13]. Negative chemical potential, Pi, reflects stable molecular structure.
Redox characteristics of BATT and NCs are illustrated in Fig. 9.
NC1 showed the smallest band gap, Eg peak-to-peak redox potential \(\Delta {E}_{\mathrm{peak}}\). Electron confinement enabled electron transfer from HOMO BATT to lower partially empty p-orbitals of Al atoms in MMT. NC1 showed the best redox characteristics [16].
In Fig. 10 TEM micrographs showed NPs in NC1 are well dispersed. Particles size ranged from 22 to 39 nm. MMT clay inorganic polymer has amorphous morphology continuous nanoscale polymeric sheets of slightly defined NPs. NC2 had tree-like morphology and large particle size 36–91 nm more aggregated than NC1.
Small particle size of NC1 confirmed large surface area/volume ratio and high Lewis basicity. Larger particle of NC2 indicated irreversible phase transition to more stable state and unsuitable DDS. It is excluded from further investigation.
Colloidal stability NC1 is established from negative zeta-potential − 26.9 mV [8] (Fig. 11).
Negative charges on NC1 protected nanoparticles against coagulation.
PXRD patterns in Fig. 12 confirmed existence of NC1 as small suspended NPs showed fine wider diffraction peaks than NC2 [17] as confirmed by crystal data.
Table SI.3 collected crystalline parameters: peak position of Miller indices h k \(\mathcal{l}\), interplanar distance (d) both experimental and calculated.
Validity of pdF cards used to simulate pXRD patterns is confirmed through calculation interplant distance, d, diffraction angle, 2θ and h k \(\mathcal{l}\) using check cell software program. Average crystalline size is calculated using Debye Scherer equation using the most intense peaks: Average particle size of NC1 and NC2 is 32 nm and 65 nm, respectively, in good agreement to those obtained from TEM micrographs. Weak intense XRD peaks of NC1 confirmed nanocrystalline size with large surface area/volume ratio. NC2 revealed moderate higher crystallinity than NC1. Structure type, geometry, crystal parameters and lattice parameters are shown in Table 5 using crystallography open database, COD, which agrees well with experimental pXRD patterns.
TGA plots related weight loss of BATT and NC1 samples on heating (Fig. 13, Table 6).
DTA plots: temperature difference between tested and reference sample versus heating temperature, t °C (Fig. 14).
Activation energy, Ea, of decomposition and thermodynamic of thermal decomposition are calculated [5], SI.2. Arrhenius type plots are represented in Fig. SI.1 for decomposition steps. Table 7 demonstrates these parameters.
Nonlinear fitting in Fig. 15 showed optimizing adsorption parameters. Nonlinear plots of % adsorbed are obtained on optimizing time, pH and initial concentration. Figure 16a: Adsorption is very fast at start and adsorption equilibrium is established rapidly. Adsorption% is limited up at 2 h. equilibrium time for completion adsorbed layer.
Small error bars (± error) confirmed accuracy of batch adsorption method on BATT on MMT. Adsorption was very fast and equilibrium is established within 2.0 h when adsorbed amount is limited with optimum pH 2 and initial concentration up to 1200 ppm for maximum adsorption MMT.
To clarify behavior of BATT, data are analyzed using several adsorption isotherms Langmuir, Freundlich, Tempkin, Dubinin–Radushkevich, D–R, Halsey, Elovich and Harkins–Jura. Empirical equations are described in SI.2. Linear regression analysis is followed in linear fitting of adsorption data [18]. High value of error functions and low value of correlation coefficients (R2) for adsorption or kinetic model indicate that this model is not fitted to adsorption isotherm [19].
Adsorption parameters are collected in Table 8.
Adsorption data did not obey Langmuir and Harkins–Jura adsorption. Linear fitting of adsorption data to Freundlich adsorption isotherm confirmed multilayer adsorption on heterogeneous surface. Heat of adsorption decreases linearly with increase surface coverage due to adsorbate–adsorbent interactions. R2 is high for D.R. model indicating suitability to describe adsorption of BATT on to MMT.
MSE is inversely proportional to correlation coefficient R2. Low value MSE and high R2 for D.R. model indicate best fitting to adsorption data.
Kinetic of adsorption informed about adsorption rate and mechanism. Adsorption data are linearly fitted to kinetic parameters pseudo-first-order-, pseudo-second-order and Elovich kinetics models. Empirical equations are shown in SI.3.
Figure 16 Variation of adsorbed amount at any time t (qt) versus t.
qt showed rapid increase with time and then qt becomes limited at 2.0 h. contact time. MMT clay intercalated large neutral (BATT) molecules into interlayer space of 2D alumina-silicate layers.
Adsorption data are best fitted to pseudo-second-order kinetic model (R2 0.999) suggesting physisorption of BATT [10]. Figure 17 shows plot t/q versus t for pseudo-second-order kinetic. Kinetic parameters K2, qe are obtained from slope and intercept of the obtained straight line (Table 9).
Small SSE confirmed validity of pseudo-second-order kinetic model for fitting adsorption data due to good agreement between qe.exp. and qe.cal. The value of \(\chi^{2}\) 0.008 less than 0.05 confirmed neglecting null hypothesis (Ho) and accepting hypothesis (H) that qe is significantly affected by contact time.
Point pH of zero point charge (pHzpc) for montmorillonite, MMT clay is determined following the method reported elsewhere [20,21,22,23,24,25,26]. Figure 18 shows clay MMT carries zero charge at pH 3. Below this pH, MMT carries positive charges of proton. Above this pH, clay carries negative charge of hydroxyl ion.
Stepwise hydration of interlayer cation in MMT enabled intercalation with positively charged molecules.
BAAT is a heterocyclic compounds contains high electron density. At pH 4, BAAT is intercalated to MMT by electron donation chemisorption with no electrostatic interaction. At pH 2, BAAT linked MMT by both chemisorption and physisorption.
Although many adsorbents are reported for removal several pollutants from aqueous solution: Cd+2 and Pb+2 [20, 21], Cr(VI) ion [22,23,24, 24,25,26,27,28,29]; pb(II) ions [30, 31], reactive dyes [32, 33], MMT clay bisorbent for adsorption of 5-[4-bromobenzylidene amino)-1, 3, 4-thiadiazole-2-thiol (BATT) is not reported yet, ecofriendly and keeping environmental safety, biodegradability, availability, etc.
MMT clays are low-cost adsorbents clays keeping environment by loading various. Good adsorbent as negative charge on framework is neutralized by adsorption positively charged cations. Clay minerals such as MMT, vermiculite, illite, caulinite and bentonite adsorbents for heavy metals are low cost than activated carbon, natural and synthetic zeolites and ion-exchange resins. MMT has excellent physicochemical properties. Clays are used: as barriers to avoid contamination of underground water and soil, preparations nanofillers and nanocomposites organic polymers. MMT clay is a bioactive glass hard solid material contains SiO2. Bioactivity depends on various proportions and available. Silicate-rich layer is produced in human fluids such as blood. MMT is safely applied in medicine for craniofacial. MMT clay is a nature-inspired layered clay mineral. Medical MMT clay is smectite phyllosilicates clays contains Al–O and Al(OH)3 octahedral (Oh) sheet sandwiched by 2 Si–O tetrahedral (Td) sheets. Triple layers sheets 2:1 layer structure are bound together by Van der Waals forces. Layer thickness 1.0 nm. Lateral layers dimensions from 100 to 1000 nm. Si+4 and Al+3cations exist in Td sheet. Al+3, Fe+3, Mg+2 Oh sheet. Si+4 substitution in both Td octahedral sheets by Al+3 ion, [1,2,3,4,5].
Conclusion
Clay montmorillonite (MMT) inorganic macromolecule is a biodegradable inert polymer carrier for biologically active and antitumor BATT heterocyclic compound. The compound showed potent antitumor activity to breast cancer and is successfully loaded on MMT clay biopolymer. Intercalation of BATT to MMT enhanced largely the electron donation capability. NC1 prepared at pH 4 showed better performance and it is followed up. Poor performance of NC2 prepared at pH 2 is attributed to self-assembled NPs. MMT is efficient adsorbent for BAAT to formulate drug delivery system for treating breast cancer. Results of thermodynamic and kinetic of adsorption suggested MMT are new promising bioadsorbent for therapeutic drugs.
Availability of data and materials
All data and materials of study are available in manuscript and SI.
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Fetouh, H.A., Abdel-Hamid, H., Zaghloul, A.A.H. et al. Formulation of promising antibacterial, anticancer, biocompatible and bioactive biomaterial as therapeutic drug delivery system for biologically active compound loaded on clay polymer. Polym. Bull. 80, 9989–10013 (2023). https://doi.org/10.1007/s00289-022-04526-2
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DOI: https://doi.org/10.1007/s00289-022-04526-2