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Evaluation of the osteoinductive potential of HDPSCs cultured on β-glycerol phosphate functionalized MWCNTs/PCL membranes for bone regeneration

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Abstract

The ideal construction and tissue substitute with adjusted characteristics and functionalities for the regeneration of bone defects are still under investigation. Current approaches have focused on the use of various biocompatible materials that have been synthesized with carbon-based materials with physicochemical characteristics that contribute to the differentiation of specific stem cell lineages. This work reports on the potential of poly-ε-caprolactone (PCL) membranes loaded with β-glycerol phosphate (β-GP) functionalized multiwall carbon nanotubes (f-MWCNTs) to induce differentiation of human dental pulp stem cells (HDPSCs) into osteoblasts. The HDPSCs were seeded on unmodified PCL + f-MWCNTs and β-GP decorated PCL + f-MWCNTs membranes, and then, physicochemical, mechanically and biologically characterized. It was observed an increase in the mechanical properties of PCL by MWCNTs addition rendering a more hydrophilic membrane those containing β-GP. Live/Dead and MTT cytotoxicity tests showed a higher number of living cells in PCL + f-MWCNTs + β-GP membranes, whereas von Kossa showed calcium deposits. After Wistar rat implantation, new bone formation was found around the critical size calvaria defects indicating that these membranes have the ability to enhance the osteodifferentiation of HDPSCs by increasing mineralization.

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Authors and Affiliations

  1. Departamento de Ingeniería Industrial, Tecnológico Nacional de México/ITS de San Luis Potosí, Capital, Carretera 57 Méx.-P.N. Km 189+100 No. 6501, C.P. 78421, San Luis Potosí, S.L.P, Mexico

    Maria Lisseth Flores-Cedillo & Lya Adlih Oros-Méndez

  2. Laboratorio de Ingeniería Tisular y Medicina Traslacional, Universidad Nacional Autónoma de México, Avenida Tenayuca-Chalmita S/N, Cuautepec Barrio Bajo, Alcaldía Gustavo A. Madero, C.P. 07239, Ciudad de México, CDMX, Mexico

    Raúl Rosales-Ibáñez

  3. Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Salvador Nava No. 2, Zona Universitaria, C.P. 78290, San Luis Potosí, S.L.P, Mexico

    Marcela Martin-del-Campo-Fierro

  4. Departamento de Patología. Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, C.P. 04510, Ciudad de México, Mexico

    Alonso Reyes-Matute

  5. Centro de Investigación Científica de Yucatán, A.C., Unidad de Materiales, Calle 43 No. 130 x 32 y 34, Mérida, Mexico

    Juan Valerio Cauich-Rodríguez

  6. Centro de Física Aplicada y Tecnología Aplicada, Departamento de Ingeniería Molecular de Materiales, Universidad Nacional Autónoma de México, Boulevard Juriquilla 3001, 76230, Juriquilla, Querétaro, Mexico

    Ángel Luis Rodríguez-Morales

  7. Bioterio de La Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios 1, Los Reyes Iztacala, Barrio de Los Árboles/Barrio de Los Héroes, C.P. 54090, Tlalnepantla de Baz, Mexico

    Maria Olga Leticia Flores-Sánchez

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  1. Maria Lisseth Flores-Cedillo
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  2. Raúl Rosales-Ibáñez
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  8. Maria Olga Leticia Flores-Sánchez
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Correspondence to Raúl Rosales-Ibáñez.

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Flores-Cedillo, M.L., Rosales-Ibáñez, R., Martin-del-Campo-Fierro, M. et al. Evaluation of the osteoinductive potential of HDPSCs cultured on β-glycerol phosphate functionalized MWCNTs/PCL membranes for bone regeneration. Polym. Bull. 79, 7229–7243 (2022). https://doi.org/10.1007/s00289-021-03721-x

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