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Targeting keratinocyte hyperproliferation, inflammation, oxidative species and microbial infection by biological macromolecule-based chitosan nanoparticle-mediated gallic acid–rutin combination for the treatment of psoriasis

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Abstract

The study deals with the formulation of biological macromolecule chitosan-based Tween 80-coated nanoparticles to deliver gallic acid and rutin into the skin for psoriasis treatment. To optimize the nanoformulations for minimum particle size and maximum possible entrapment efficiency (dependent variables), 32 full factorial design was used to optimize the formulation. Concentration of Tween 80 and chitosan were independent variables. The optimized chitosan nanoformulation of gallic acid, rutin and their combination was explored against psoriasis using in vitro methods and HaCaT cell line. The results indicated excellent entrapment of drug in the chitosan polymer and Higuchi model of drug release. The optimized encapsulated Tween 80-coated chitosan nanoparticles containing combination of gallic acid and rutin showed the reduced keratinocyte hyperproliferation, antioxidant, anti-inflammatory and antimicrobial activity even better than the chitosan nanoparticles containing gallic acid and rutin individually in the nanoparticles. Anti-psoriasis like activity of Tween 80-coated nanoformulations of gallic acid may be attributed to faster penetration of the drug by increased permeation and fusion of drug molecules. Thus, the present in vitro investigation indicates that chitosan-based nanoformulations hold promising potential in the treatment of psoriasis. The activity was increased once we combine gallic acid and rutin.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Chaudhary Bansi Lal University, Bhiwani, Haryana, India

    Amarjeet Shandil, Monu Yadav & Aakash Deep

  2. Shri Baba Mastnath Institute of Pharmaceutical Science and Research, Rohtak, Haryana, India

    Nidhi Sharma

  3. Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida, 201313, India

    Kalpana Nagpal

  4. Department of Pharmaceutical Science, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India

    Deepak Kumar Jindal & Sunil Kumar

  5. Pharmacology Division, UIPS, Panjab University, Chandigarh, 160014, India

    Monu Yadav

Authors
  1. Amarjeet Shandil
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  2. Monu Yadav
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  3. Nidhi Sharma
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  4. Kalpana Nagpal
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  5. Deepak Kumar Jindal
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  6. Aakash Deep
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  7. Sunil Kumar
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Correspondence to Monu Yadav or Kalpana Nagpal.

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Shandil, A., Yadav, M., Sharma, N. et al. Targeting keratinocyte hyperproliferation, inflammation, oxidative species and microbial infection by biological macromolecule-based chitosan nanoparticle-mediated gallic acid–rutin combination for the treatment of psoriasis. Polym. Bull. 77, 4713–4738 (2020). https://doi.org/10.1007/s00289-019-02984-9

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  • DOI: https://doi.org/10.1007/s00289-019-02984-9

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