Skip to main content
Log in

Alginate/gelatin crosslinked system through Maillard reaction: preparation, characterization and biological properties

  • Original Paper
  • Published:
Polymer Bulletin Aims and scope Submit manuscript

Abstract

Maillard reaction (MR) was studied in aqueous model systems containing gelatin and sodium alginate, which were heat treated for different pH (7, 8, 9, 10 and 11) at three temperatures (70, 80 and 90 °C). Some indicators were used to evaluate this reaction:degree of crosslinking, release of gelatin, free amino groups and browning intensities. The results indicated that alginate/gelatin crosslinked by MR showed an increase of degree of crosslinking as the pH and temperatures were increasing while release of gelatin decreased. Furthermore, samples prepared at a high temperature exhibited stronger browning intensity owing to the formation of Maillard reaction products (MRPs). The obtained materials were analyzed by FTIR and XRD. The antioxidant ability by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and power reducing, as well as the anti-inflammatory activity were investigated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12

Similar content being viewed by others

References

  1. Wang LF, Shankar S, Rhim JW (2017) Properties of alginate-based films reinforced with cellulose fibers and cellulose nanowhiskers isolated from mulberry pulp. Food Hydrocoll 63:201–208

    Article  CAS  Google Scholar 

  2. Dong Z, Wang Q, Du Y (2006) Alginate/gelatin blend films and their properties for drug controlled release. J Membr Sci 280:37–44

    Article  CAS  Google Scholar 

  3. Aadil KR, Prajapati D, Jha H (2016) Improvement of physcio-chemical and functional properties of alginate film by Acacia lignin. Food Packag 10:25–33

    Article  Google Scholar 

  4. Mandal B, Ray SK (2013) Synthesis of interpenetrating network hydrogel from poly(acrylic acid-co-hydroxyethyl methacrylate) and sodium alginate: modeling and kinetics study for removal of synthetic dyes from water. Carbohydr Polym 98:257–269

    Article  CAS  Google Scholar 

  5. Pawar SN, Edgar KJ (2012) Alginate derivatization: a review of chemistry, properties and applications. Biomaterials 33:3279–3305

    Article  CAS  Google Scholar 

  6. Hattori M, Ogino A, Nakai H, Takahashi K (1996) Functional improvement of alginic acid by conjugating with β-lactoglobulin. J Agric Food Chem 61:1171–1176

    CAS  Google Scholar 

  7. Razzak MA, Kim M, Chung D (2016) Elucidation of aqueous interactions between fish gelatin and sodium alginate. Carbohydr Polym 148:181–188

    Article  CAS  Google Scholar 

  8. Ertesvåg H, Valla S, Skjåk-bræk G (2009) Enzymatic Alginate Modification. In: Rehm BHA (ed) Alginates: biology and applications. Springer, Berlin Heidelberg, pp 95–115

    Chapter  Google Scholar 

  9. Boanini E, Rubini K, Panzavolta S, Bigi A (2010) Chemico-physical characterization of gelatin films modified with oxidized alginate. Acta Biomater 6:383–388

    Article  CAS  Google Scholar 

  10. Nguyen VT, Ko SC, Oh GW, Heo SY, Jeon YJ, Park WS, Choi IW, Choi SW, Jung WK (2016) Anti-inflammatory effects of Sodium alginate/gelatine porous scaffolds merged with fucoidan in murine microglial BV2 cells. Int J Biol, Macromol

    Google Scholar 

  11. Karim AA, Bhat R (2009) Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocoll 23:563–576

    Article  CAS  Google Scholar 

  12. Devi N, Kakati DK (2013) Smart porous microparticles based on gelatin/sodium alginate polyelectrolyte complex. J Food Eng 117:193–204

    Article  CAS  Google Scholar 

  13. Hwang IG, Kim HY, Woo KS, Lee J, Jeong HS (2011) Biological activities of Maillard reaction products (MRPs) in a sugar–amino acid model system. Food Chem 26:221–227

    Article  Google Scholar 

  14. Nursten H (2005) The Maillard reaction: chemistry, biochemistry and implications. Royal Society of Chemistry, Cambridge, pp 1–208

    Google Scholar 

  15. Gerrard JA (2002) New aspects of an AGEing chemistry-recent developments concerning the Maillard reaction. Aust J Chem 55:299–310

    Article  CAS  Google Scholar 

  16. Hiller B, Lorenzen PC (2010) Functional properties of milk proteins as affected by Maillard reaction induced oligomerisation. Food Res Int 43:1155–1166

    Article  CAS  Google Scholar 

  17. Liu Q, Li J, Kong B, Jia N, Li P (2014) Antioxidant capacity of Maillard reaction products formed by a porcine plasma protein hydrolysate-sugar model system as related to chemical characteristics. J Food Sci Biotechnol 23:33–41

    Article  CAS  Google Scholar 

  18. Vhangani LN, Wyk JV (2013) Antioxidant activity of Maillard reaction products (MRPs) derived from fructose–lysine and ribose–lysine model systems. Food Chem 137:92–98

    Article  CAS  Google Scholar 

  19. Virág D, Kiss A, Forgó P, Csutorás C, Molnár S (2013) Study on Maillard-reaction driven transformations and increase of antioxidant activity in lysine fortified biscuits. Microchem J 107:172–177

    Article  Google Scholar 

  20. Jiang Z, Brodkorb A (2012) Structure and antioxidant activity of Maillard reaction products from α-lactalbumin and β-lactoglobulin with ribose in an aqueous model system. Food Chem 133:960–968

    Article  CAS  Google Scholar 

  21. Gu FL, Kim JM, Abbas S, Zhang XM, Xia SQ, Chen ZX (2010) Structure and antioxidant activity of high molecular weight Maillard reaction products from casein–glucose. Food Chem 120:505–511

    Article  CAS  Google Scholar 

  22. Labuza TP, Reineccius GA, Monnier VM, O’Brien J, Baynes JW (1994) Maillard reactions in chemistry, food and health. R Soc Chem, Cambridge, pp 1–435

    Google Scholar 

  23. Zhuang Y, Sun L (2013) Antioxidant activity of maillard reaction products from lysine-glucose model system as related to optical property and copper (II) binding ability. Af J Biotechnol 10:6784–6793

    Google Scholar 

  24. Bastos DM, Monaro É, Siguemoto É, Séfora M (2012) Maillard reaction products in processed food: pros and cons, food industrial processes-methods and equipment. INTECH Open Access Publisher, Rijeka, pp 281–300

    Google Scholar 

  25. Liu Q, Li J, Kong B, Li P, Xia X (2014) Physicochemical and antioxidant properties of Maillard reaction products formed by heating whey protein isolate and reducing sugars. Int J Dairy Technol 67:220–228

    Article  CAS  Google Scholar 

  26. Ofner CM, Bubnis WA (1996) Chemical and swelling evaluations of amino group crosslinking in gelatin and modified gelatine matrices. Pharm Res 13:1821–1827

    Article  CAS  Google Scholar 

  27. Hoffmann B, Seitz D, Mencke A, Kokott A, Ziegler G (2009) Glutaraldehyde and oxidised dextran as crosslinker reagents for chitosan-based scaffolds for cartilage tissue engineering. J Mater Sci 20:1495–1503

    CAS  Google Scholar 

  28. Laroque D, Inisan C, Berger C, Vouland É, Dufossé L, Guérard F (2008) Kinetic study on the Maillard reaction. Consideration of sugar reactivity. Food Chem 111:1032–1042

    Article  CAS  Google Scholar 

  29. Norajit K, Kim KM, Ryu GH (2010) Comparative studies on the characterization and antioxidant properties of biodegradable alginate films containing ginseng extract. J Food Eng 98:377–384

    Article  CAS  Google Scholar 

  30. Alhakmani F, Kumar S, Khan SA (2013) Estimation of total phenolic content, in vitro antioxidant and anti-inflammatory activity of flowers of M oringa oleifera. Asian Pac J Trop Biomed 3:623–627

    Article  CAS  Google Scholar 

  31. Bubnis WA, Ofner CM (1992) The determination of ε-amino groups in soluble and poorly soluble proteinaceous materials by a spectrophotometric method using trinitrobenzenesulfonic acid. Anal Biochem 133:129–133

    Article  Google Scholar 

  32. Ahmad M, Benjakul S (2011) Characteristics of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as influenced by acid pretreatment and extraction time. Food Hydrocoll. 25:381–388

    Article  CAS  Google Scholar 

  33. Rbii K, Violleau F, Brambati N, Buchert AM, Surel O (2011) Decrease of available lysine in thermally treated gelatin followed by LC-UV: influence on molar mass and ability to helixes formation. Food Hydrocoll 25:1409–1412

    Article  CAS  Google Scholar 

  34. Zandi M, Mirzadeh H, Mayer C (2017) Effects of concentration, temperature, and pH on chain mobility of gelatin during the early stages of gelation. Iran Polym J 12:861–870

    Google Scholar 

  35. Gómez-Mascaraque LG, Méndezc JA, Fernández-Gutiérrez M, Vázquez B, Román JS (2014) Oxidized dextrins as alternative crosslinking agents for polysaccharides: application to hydrogels of agarose-chitosan. Acta Biomater 10:798–811

    Article  Google Scholar 

  36. Carabasa-Giribet M, Ibarz-Ribas A (2000) Kinetics of colour development in aqueous glucose systems at high temperatures. J Food Eng 44:181–189

    Article  Google Scholar 

  37. Decourcelle N, Sabourin C, Dauer G, Guérard F (2010) Effect of the Maillard reaction with xylose on the emulsifying properties of a shrimp hydrolysate (Pandalus borealis). Food Res Int 43:2155–2160

    Article  CAS  Google Scholar 

  38. Riquelme N, Díaz-Calderón P, Enrione J, Matiacevich S (2015) Effect of physical state of gelatin-plasticizer based films on to the occurrence of Maillard reactions. Food Chem 175:478–484

    Article  CAS  Google Scholar 

  39. Kim JS, Lee YS (2009) Enolization and racemization reactions of glucose and fructose on heating with amino-acid enantiomers and the formation of melanoidins as a result of the Maillard reaction. Food Chem 36:465–474

    CAS  Google Scholar 

  40. Li C, Liu F, Gong Y, Wang Y, Xu H, Yuan F, Gao Y (2014) Investigation into the Maillard reaction between ɛ-polylysine and dextran in subcritical water and evaluation of the functional properties of the conjugates. LWT Food Sci Technol 57:612–617

    Article  CAS  Google Scholar 

  41. Ramonaityte DT, Keršiene M, Adamsb A, Tehrani KA, Kimpe ND (2009) The interaction of metal ions with Maillard reaction products in a lactose-glycine model system. Food Res Int 42:331–336

    Article  CAS  Google Scholar 

  42. Li Y, Lua F, Luo C, Chen Z, Mao J, Shoemaker C, Zhong F (2009) Functional properties of the Maillard reaction products of rice protein with sugar. Food Chem 117:69–74

    Article  CAS  Google Scholar 

  43. Liu Q, Kong B, Han J, Sun C, Li P (2014) Structure and antioxidant activity of whey protein isolate conjugated with glucose via the Maillard reaction under dry-heating conditions. Food Struct 1:145–154

    Article  Google Scholar 

  44. Carbonaro M, Nucara A (2010) Secondary structure of food proteins by Fourier transform spectroscopy in the mid-infrared region. Amino Acids 38:679–690

    Article  CAS  Google Scholar 

  45. Farhat IA, Orset S, Moreau P, Blanshard JMV (1998) FTIR study of hydration phenomena in protein-sugar systems. J Colloid Interface Sci 207:200–208

    Article  CAS  Google Scholar 

  46. Kareb O, Gomaa A, Champagne CP, Jean J, Aïder M (2017) Electro-activation of sweet defatted whey: impact on the induced Maillard reaction products and bioactive peptides. Food Chem 221:590–598

    Article  CAS  Google Scholar 

  47. Yaylayan V, Ismail A, Huyghues-Despointe A (1994) Investigation of the acyclic forms of reducing sugars and Amadori products by FTIR spectroscopy. Royal Society of Chemistry, Cambridge, UK, pp 69–74

    Google Scholar 

  48. Kuila SB, Ray SK (2014) Dehydration of dioxane by pervaporation using filled blend membranes of polyvinyl alcohol and sodium alginate. Carbohydr Polym 101:1154–1165

    Article  CAS  Google Scholar 

  49. Sarker B, Papageorgiou DG, Silva R, Zehnder T, Gul-E-Noor F, Bertmer M et al (2014) Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties. J Mater Chem B 2:1470–1482

    Article  CAS  Google Scholar 

  50. Asma C, Meriem E, Mahmoud B, Djaafer B (2014) Physicochemical characterization of gelatin-cmc composite edibles films from polyion-complex hydrogels. J Chil Chem Soc 1:2279–2283

    Article  Google Scholar 

  51. Lertittikul W, Benjakul S, Tanaka M (2007) Characteristics and antioxidative activity of Maillard reaction products from a porcine plasma protein-glucose model system as influenced by pH. Food Chem 100:669–677

    Article  CAS  Google Scholar 

  52. Phisut N, Jiraporn B (2013) Characteristics and antioxidant activity of Maillard reaction products derived from chitosan-sugar solution. Int Food Res J 20:1077–1085

    CAS  Google Scholar 

  53. Amarowicz R (2009) Antioxidant activity of Maillard reaction products. Eur J Lipid Sci Technol 111:109–111

    Article  CAS  Google Scholar 

  54. Huang X, Tu Z, Xiao H, Wang H, Zhang L, Hu Y, Zhang Q, Niu P (2012) Characteristics and antioxidant activities of ovalbumin glycated with different saccharides under heat moisture treatment. Food Res Int 48:866–872

    Article  CAS  Google Scholar 

  55. Manzocco L, Calligaris S, Mastrocola D, Nicoli MC, Lerici CR (2000) Review of non-enzymatic browning and antioxidant capacity in processed foods. Trends Food Sci Technol 11:340–346

    Article  CAS  Google Scholar 

  56. Mirshafiey A, Rehm BHA (2009) Alginate and its comonomer mannuronic acid: medical relevance as drugs. In: Rehm BHA (ed) Alginates: Biology and Applications. Springer, Berlin Heidelberg, pp 229–260

    Chapter  Google Scholar 

  57. Kitts DD, Chen XM, Jing H (2012) Demonstration of antioxidant and anti-inflammatory bioactivities from sugar-amino acid maillard reaction products. J Agric Food Chem 60:6718–6727

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Chetouani Asma.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khadidja, L., Asma, C., Mahmoud, B. et al. Alginate/gelatin crosslinked system through Maillard reaction: preparation, characterization and biological properties. Polym. Bull. 74, 4899–4919 (2017). https://doi.org/10.1007/s00289-017-1997-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00289-017-1997-z

Keywords

Navigation