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Azo-polymers modified with nucleobases and their interactions with DNA molecules

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Abstract

The photo-fluidization process which is specific for azo-materials opens a new perspective for their use in the field of molecules nano manipulation at the surface of the azo polymer films. This is possible considering that in the case of the UV irradiation from a polarized laser source the azo material has an unidirectional flow. Here, we investigated the structuring phenomena occurring on the surface of the azo-polysiloxanes films modified with nucleobases, upon UV irradiation. Measurements of topography and adhesive forces between polymeric substrates and a hydrophilic probe have been done by atomic force microscopy (AFM). The response of the material upon irradiation has been investigated also by using UV−VIS spectroscopy. This method allowed us to draw the photo-isomerization and relaxation curves. Also, preliminary tests were conducted to determine the capacity of the film surface to immobilize DNA molecules.

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Acknowledgment

The roumanian authors want to thank to Roumanian Ministery of Education Research and Inovation, for financial support of this research (CNCSIS Idei-356/2008).

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Author notes

  1. Elena Taran

    Present address: Australian Institute for Bioengineering & Nanotechnology, Australian National Fabrication Facility, The University of Queensland, Brisbane, QLD, 4072, Australia

Authors and Affiliations

  1. Department of Natural and Synthetic Polymers, “Gheorghe Asachi” Technical University of Iasi, Bd. Mangeron 71, 700050, Iasi, Romania

    Elena-Luiza Epure, Ioana Andreea Moleavin & Nicolae Hurduc

  2. School of Chemical Engineering, University of Queensland, Brisbane, QLD, 4072, Australia

    Elena Taran & Anh V. Nguyen

  3. Department of Viral Glycoproteins, Institute of Biochemistry, Splaiul Independentei 296, Sector 6, Bucharest, Romania

    Norica Nichita

Authors
  1. Elena-Luiza Epure
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  2. Ioana Andreea Moleavin
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  3. Elena Taran
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  4. Anh V. Nguyen
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  5. Norica Nichita
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  6. Nicolae Hurduc
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Correspondence to Nicolae Hurduc.

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Epure, EL., Moleavin, I.A., Taran, E. et al. Azo-polymers modified with nucleobases and their interactions with DNA molecules. Polym. Bull. 67, 467–478 (2011). https://doi.org/10.1007/s00289-010-0436-1

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  • DOI: https://doi.org/10.1007/s00289-010-0436-1

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