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Encapsulation of BSA in polylactic acid–hyperbranched polyglycerol conjugate nanoparticles: preparation, characterization, and release kinetics

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Abstract

Nanoparticles based on an amphiphilic copolymer with polylactic acid (PLA) grafted onto hyperbranched polyglycerol (HPG) were prepared by the use of BSA as a model protein. The characteristics of the nanoparticles were evaluated using particle size analyzer, transmission electron microscopy, and X-ray photoelectron spectroscopy. The secondary structure of BSA released from nanoparticles were analysed by circular dichroism experiments. Cell viability of nanoparticles was also evaluated by using NIH 3T3 cells. The mechanism of BSA release was studied by fitting experimental data to three model equations. Results indicated that BSA diffusion and the polymeric relaxation jointly governed the overall release process. The detailed analysis of BSA release was performed using the first-order kinetic model equation, which gave a good fit to the experimental release data. The influence of different copolymer structures and BSA loading capacities on release profiles were also evaluated for the potential of using nanoparticles as controlled release protein delivery systems.

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Acknowledgments

This work was supported in part by the National Natural Science Foundation of China (Grant No. 20804021), the Natural Science Foundation of Tianjin (Grant No. 08JCYBJC00300) and the Ph.D. Programs foundation for new teachers of Ministry of Education of China (No. 200800551030).

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Authors and Affiliations

  1. Key Laboratory of Functional Polymer Materials of Ministry Education, Institute of Polymer Chemistry, Nankai University, Tianjin, 300071, China

    Xiujun Gao, Xinge Zhang, Xuejiao Zhang, Cui Cheng, Zhen Wang & Chaoxing Li

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  1. Xiujun Gao
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  2. Xinge Zhang
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  3. Xuejiao Zhang
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Correspondence to Xinge Zhang or Chaoxing Li.

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Gao, X., Zhang, X., Zhang, X. et al. Encapsulation of BSA in polylactic acid–hyperbranched polyglycerol conjugate nanoparticles: preparation, characterization, and release kinetics. Polym. Bull. 65, 787–805 (2010). https://doi.org/10.1007/s00289-010-0273-2

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  • DOI: https://doi.org/10.1007/s00289-010-0273-2

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