Abstract
Fe-hydrogenase-specific degenerate primers were used in polymerase chain reactions with single-stranded Scenedesmus obliquus cDNA as template. A resulting 435-bp fragment was used to screen a cDNA library from S. obliquus. A 1.6-kb cDNA clone, containing the 3′-end of an open reading frame, was obtained. Its deduced amino acid sequence contains the conserved H-cluster motives unique for Fe-hydrogenases. Any other FeS-clusters seem to be absent. Phylogenetically, the enzyme is closely related to the Trichomonas vaginalis Fe-hydrogenase. Northern blot analysis shows that the Fe-hydrogenase is constitutively expressed in S. obliquus. Southern blot analysis of plastidic, mitochondrial, and nuclear DNA reveals that the enzyme is a single-copy gene encoded in the nucleus. Effects of transcriptional and translational inhibitors on H2-metabolism reveal the involvement of a chloroplast-encoded protein.
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Received: 29 September 2000 / Accepted: 30 October 2000
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Wünschiers, R., Stangier, K., Senger, H. et al. Molecular Evidence for a Fe-Hydrogenase in the Green Alga Scenedesmus obliquus . Curr Microbiol 42, 353–360 (2001). https://doi.org/10.1007/s002840010229
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DOI: https://doi.org/10.1007/s002840010229