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Green-Synthesized Silver Nanoparticles in the Prevention of Multidrug-Resistant Proteus mirabilis Infection and Incrustation of Urinary Catheters BioAgNPs Against P. mirabilis Infection

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Abstract

This study aimed to assess the activity of AgNPs biosynthesized by Fusarium oxysporum (bio-AgNPs) against multidrug-resistant uropathogenic Proteus mirabilis, and to assess the antibacterial activity of catheters coated with bio-AgNPs. Broth microdilution and time-kill kinetics assays were used to determine the antibacterial activity of bio-AgNPs. Catheters were coated with two (2C) and three (3C) bio-AgNPs layers using polydopamine as crosslinker. Catheters were challenged with urine inoculated with P. mirabilis to assess the anti-incrustation activity. MIC was found to be 62.5 µmol l-1, causing total loss of viability after 4 h and bio-AgNPs inhibited biofilm formation by 76.4%. Catheters 2C and 3C avoided incrustation for 13 and 20 days, respectively, and reduced biofilm formation by more than 98%, while the pristine catheter was encrusted on the first day. These results provide evidence for the use of bio-AgNPs as a potential alternative to combat of multidrug-resistant P. mirabilis infections.

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Funding

This study was financed by the Coordination for the Improvement of Higher Education Personnel—Brazil (CAPES) Finance Code 001.

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Authors and Affiliations

  1. Laboratory of Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Rodovia Celso Garcia Cid PO-BOX 6001, Londrina, 86051-980, Brazil

    Gustavo Issamu Asai Saikawa, Gustavo Henrique Migliorini Guidone & Sergio Paulo Dejato Rocha

  2. Laboratory of Microbial Biotechnology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil

    Sandriele Aparecida Noriler, Guilherme Fonseca Reis & Admilton Gonçalves de Oliveira

  3. Laboratory of Electron Microscopy and Microanalysis, State University of Londrina, Londrina, Brazil

    Admilton Gonçalves de Oliveira

  4. Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, State University of Londrina, Londrina, Brazil

    Gerson Nakazato

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  1. Gustavo Issamu Asai Saikawa
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  2. Gustavo Henrique Migliorini Guidone
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  3. Sandriele Aparecida Noriler
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  7. Sergio Paulo Dejato Rocha
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Contributions

All authors contributed to the study conception and design. GIAS, GHMG, SAN, GFR and AGOd: Material preparation, data collection and analysis were performed. AGdO, GN and SPDR: The first draft of the manuscript was written, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Sergio Paulo Dejato Rocha.

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This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of University State of Londrina (CEP-UEL 1.590.120).

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Saikawa, G.I.A., Guidone, G.H.M., Noriler, S.A. et al. Green-Synthesized Silver Nanoparticles in the Prevention of Multidrug-Resistant Proteus mirabilis Infection and Incrustation of Urinary Catheters BioAgNPs Against P. mirabilis Infection. Curr Microbiol 81, 100 (2024). https://doi.org/10.1007/s00284-024-03616-w

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