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Genome-Based Reclassification of Anoxybacillus geothermalis Filippidou et al. 2016 as a Later Heterotypic Synonym of Anoxybacillus rupiensis Derekova et al. 2007

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Abstract

In this study, our aim was to elucidate the relationship between Anoxybacillus rupiensis DSM 17127T and Anoxybacillus geothermalis GSsed3T through whole-genome phylogenetic analysis. The obtained 16S rRNA gene sequence from the genome of A. rupiensis DSM 17127T exhibited a 99.8% similarity with A. geothermalis GSsed3T. In the phylogenetic trees constructed using whole-genome sequences and 16S rRNA gene sequences, A. rupiensis DSM 17127T and A. geothermalis GSsed3T were observed to form a clade, indicating a close relationship between them. Moreover, the average amino acid identity, average nucleotide identity, and digital DNA–DNA hybridization values calculated between A. rupiensis DSM 17127T and A. geothermalis GSsed3T exceeded the threshold values typically used for species demarcation. Furthermore, the phylogenomic analysis based on the core genome of the strains in question provided additional support for the formation of a monophyletic clade by A. rupiensis DSM 17127T and A. geothermalis GSsed3T. Most phenotypic and chemotaxonomic features between both strains were almost identical except for a few exceptions. These findings suggest that both strains should be classified as belonging to the same species, and we propose that A. geothermalis GSsed3T is a later heterotypic synonym of A. rupiensis DSM 17127T.

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Funding

This study was supported by Karadeniz Technical University (KTU BAP FAT-2019-7822).

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Kadriye Inan Bektas, Aleyna Nalcaoglu & Halil İbrahim Guler

  2. Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Sabriye Canakci & Ali Osman Belduz

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  1. Kadriye Inan Bektas
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  2. Aleyna Nalcaoglu
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Contributions

KIB designed the study. KIB, AOB, and SC performed genome analysis. KIB, HIB, and AN analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Kadriye Inan Bektas.

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Inan Bektas, K., Nalcaoglu, A., Guler, H.İ. et al. Genome-Based Reclassification of Anoxybacillus geothermalis Filippidou et al. 2016 as a Later Heterotypic Synonym of Anoxybacillus rupiensis Derekova et al. 2007. Curr Microbiol 81, 102 (2024). https://doi.org/10.1007/s00284-024-03615-x

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