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Genome-based reclassification of Anoxybacillus salavatliensis Cihan et al. 2011 as a later heterotypic synonym of Anoxybacillus gonensis Belduz et al. 2003

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Abstract

In the present study, we aim to clarify the taxonomic positions of Anoxybacillus salavatliensis DSM 22626T and Anoxybacillus gonensis G2T by using whole genome phylogenetic analysis, biochemical and chemotaxonomic characteristics. The genome sequences of A. salavatliensis DSM 22626T was not available in any database, so it was sequenced in this study. In phylogenetic trees drawn using whole genome sequences and 16S rRNA gene sequences, A. salavatliensis DSM 22626T and A. gonensis G2T clade together and showed high sequence similarity (99.3%) based on 16S rRNA gene. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values between A. salavatliensis DSM 22626T and A. gonensis G2T were found to be greater than the threshold values for species demarcation. Further, the phylogenomic analysis based on the core genome of the strains under study confirmed that A. salavatliensis DSM 22626T and A. gonensis G2T formed a monophyletic clade. Most phenotypic and chemotaxonomic features between both strains were almost identical except for a few exceptions. The present results show that A. salavatliensis DSM 22626T is a later heterotypic synonym of A. gonensis G2T.

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Acknowledgements

This study was supported by Karadeniz Technical University (KTU BAP FAT-2019-7822).

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Authors and Affiliations

  1. Department of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Kadriye Inan Bektas & Halil İbrahim Guler

  2. Department of Biology, Faculty of Science, Karadeniz Technical University, 61080, Trabzon, Turkey

    Sabriye Canakci & Ali Osman Belduz

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  1. Kadriye Inan Bektas
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  2. Halil İbrahim Guler
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  3. Sabriye Canakci
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  4. Ali Osman Belduz
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Contributions

KIB designed the study. KIB, HIG and SC performed genome analysis and analysed the data. KIB, HIG and AOB performed the phenotypic and chemotaxonomic analysis. KIB wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Kadriye Inan Bektas.

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Inan Bektas, K., Guler, H.İ., Canakci, S. et al. Genome-based reclassification of Anoxybacillus salavatliensis Cihan et al. 2011 as a later heterotypic synonym of Anoxybacillus gonensis Belduz et al. 2003. Antonie van Leeuwenhoek 116, 415–423 (2023). https://doi.org/10.1007/s10482-023-01813-4

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