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Halotolerant Bacteria from Genus Nesterenkonia sandarakina VSA9 as a Potential Polyhydroxyalkanoate Producer

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Abstract

Nesterenkonia sandarakina VSA9 pigmented bacteria isolated from Sargassum is being reported to produce polyhydroxyalkanoates (PHA) deduced through detecting the presence of pha C gene using the molecular method. The PHA synthase gene was of type I which has been concluded from the phylogenetic tree and multiple sequence analysis. The amino acid analysis of pha C gene confirms the involvement of the lipase box having a sequence of G–Y–C–I–G–G with cysteine as the active center of the PHA synthase. Homology modeling predicted the 3D protein structure which is similar to the PHA synthase of Chromobacterium sp. USM2. The solvent extract of N. sandarakina VSA9 showed the presence of Carotenoid compound with maximum wavelength at 475 nm. The study’s findings could have far-reaching implications, contributing to advancements in the biotechnology, industrial processes, and sustainable practices. The simultaneous production of carotenoids and PHAs by N. sandarakina VSA9 presents exciting opportunities for the development of innovative and environmentally friendly applications.

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The data that support the findings of the study are available on request from the corresponding author.

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Acknowledgements

The authors are thankful to Director, HOD of Biological Oceanography Division for providing Laboratory facilities. The authors would like to thank Dr. Supriya Tilvi and Miss Shambhavi Naik for providing UV–visible spectrophotometer facility. This is NIO Contribution No. 7159.

Funding

Department of Biotechnology, Govt. of India, New Delhi for providing fellowship. CSIR-National Institute of Oceanography for financial support from OLP 2005 project.

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Authors and Affiliations

  1. Microbial Ecology Laboratory, Biological Oceanography Division, CSIR-National Institute of Oceanography, Raj Bhavan Road, Dona Paula, Goa, 403004, India

    Harshada T. Kankonkar & Rakhee S. Khandeparker

  2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India

    Harshada T. Kankonkar & Rakhee S. Khandeparker

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  1. Harshada T. Kankonkar
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HK conceived the study. RK guided the experiments which were carried out. HK searched database for articles and wrote the manuscript. RK critically analyzed the data and revised the manuscript. The authors read and approved the Manuscript.

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Correspondence to Rakhee S. Khandeparker.

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Kankonkar, H.T., Khandeparker, R.S. Halotolerant Bacteria from Genus Nesterenkonia sandarakina VSA9 as a Potential Polyhydroxyalkanoate Producer. Curr Microbiol 81, 53 (2024). https://doi.org/10.1007/s00284-023-03569-6

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