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Ochrobactrum chromiisoli sp. nov., Isolated from Chromium-Contaminated Soil

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Abstract

A Gram-stain-negative, non-spore-forming, flagellated, motile, aerobic, rod-shaped bacteria strain, designated YY2XT, was isolated from chromium-contaminated soil. Phylogenetic analysis based on 16S rRNA gene, recA gene, and whole genome indicated that the strain represented a new member of the genus Ochrobactrum, family Brucellaceae, class Alphaproteobacteria. The phylogenetic trees based on 16 s rRNA gene, revealed that Falsochrobactrum ovis DSM26720T (96.7%), Ochrobactrum gallinifaecis DSM15295T (96.2%), and Pseudochrobactrum asaccharolyticum DSM25619T (96.2%) are the most closely related phylogenetic neighbors of strain YY2XT. The draft genome of YY2XT was approximately 4,650,646 bp in size with a G + C content of 53.0 mol%. Average nucleotide identity and digital DNA–DNA hybridization values among strain YY2XT and the selected Brucellaceae species were 71.4–83.1% and 13.5–42.7%, which are below the recommended cut-off values for species delineation. Growth of strain YY2XT occurred within pH 5–10 (optimum, pH 7–8), 4 ℃–42 °C (optimum, 30 °C), and NaCl concentrations of 0.0–6.0% (optimum, 1.0%). Major quinone system was ubiquinone 10, the major fatty acids were C16:0, C18:1ω7c, and C16:1ω7c and the major polyamines were spermidine and putrescine. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and four undefined lipids. On the basis of the phenotypic, genotypic and chemotaxonomic traits, strain YY2XT was considered to represent a novel species of the genus Ochrobactrum, for which the name Ochrobactrum chromiisoli sp. nov. is proposed. The type strain is YY2XT (= CCTCC AB 2023035T = JCM 36000T).

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Acknowledgements

We are grateful to Prof. Bernhard Schink (Fachbereich Biologie, Universitaet Konstanz) for his kindly help in the scientific naming of strain YY2XT. We are grateful to Yajun Lv (CCTCC), Luan Yan (CCTCC), Liu Jia (CCTCC), Yasuo Amano (JCM) and Takao Iino (JCM)) for conservation YY2XT.

Funding

This study was financially supported by the Major Scientific and Technological Achievements Transformation Project of Sichuan Province (Grant No. 2022ZHCG0128), Science and technology project of Sichuan Dazhou (2021CDDZ-19) and the Enzyme Resources Sharing and Service Platform of Sichuan Province (Grant No. 2021JDS0020).

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  1. Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, People’s Republic of China

    Yi Yang, Zhe Xu, Li Yang, Meng-yao Hu, Guang-yang Jiang, Jia Chen, Yi-chen Yang & Yongqiang Tian

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Contributions

YY isolated strain, completed trials and wrote the main manuscript. ZX, LY and MYH provided methodological guidance. YQT, GYJ and ZX revised the manuscript. JC and YCY were involved in charting. All authors contributed to the article and approved the submitted version.

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Correspondence to Yongqiang Tian.

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Yang, Y., Xu, Z., Yang, L. et al. Ochrobactrum chromiisoli sp. nov., Isolated from Chromium-Contaminated Soil. Curr Microbiol 81, 50 (2024). https://doi.org/10.1007/s00284-023-03562-z

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