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Thiobencarb Degradation by Pseudomonas sp. Th1 and Cupriavidus oxalaticus Th2 Isolated from Soil

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Abstract

Thiobencarb has been extensively applied for weed control, resulting in severe environmental problems. In this study, thiobencarb degradation in liquid media and in soil by two bacterial strains, Pseudomonas sp. Th1 and Cupriavidus oxalaticus Th2, was investigated. Both bacterial isolates utilized the compound as a sole carbon, nitrogen and sulfur source. The utilization rates of thiobencarb by Pseudomonas sp. Th1 and C. oxalaticus Th2 in a liquid mineral medium were 1.02 ± 0.11 and 0.80 ± 0.07 µM/h at 100 µM, respectively. The determination of degradation and bacterial growth rates kinetics showed that the rates for pure thiobencarb followed the Michaelis–Menten model; meanwhile, the rates for thiobencarb in a commercial herbicide fitted well with the Edwards model. Their degradation by the mixed culture of both strains reduced the accumulation of intermediate products, including S-4-chlorobenzyl ethylthiocarbamate and 4-chlorobenzyl mercaptan, in media. The degradation by the mixed culture of these bacteria immobilized in rice straw was significantly higher than those of their free counterparts when determining in a packed bed bioreactor (P < 0.05). In addition, the inoculation of the mixed bacterial culture in soil significantly enhanced the degradation performance for both thiobencarb and propanil in a commercial herbicide. This study elucidates the differences in biodegradation of pure thiobencarb and thiobencarb in an herbicide.

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Acknowledgements

We are also grateful to the anonymous reviewers and the Executive Editor of Current Microbiology, whose suggestions helped to improve this manuscript.

Funding

This study was supported by the Vietnamese Ministry of Education and Training for the scientific theme (Code: B2022.SPD.04). The authors would like to thank them for all supports.

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Authors and Affiliations

  1. Dong Thap University, 783 Pham Huu Lau Street, Cao Lanh City, Dong Thap Province, Vietnam

    Ha Danh Duc & Nguyen Thi Oanh

  2. Can Tho University of Technology, 256 Nguyen Van Cu Street, An Hoa Ward, Ninh Kieu District, Can Tho City, Vietnam

    Nguyen Thi Mai Khanh

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  1. Ha Danh Duc
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Contributions

HDD: designed the work plan and performed the experiments, prepared figures and tables. NTO and HDD: wrote this manuscript, reviewed and approved the final draft and approved the final version. NTMK: collected soil samples and prepared liquid media. All authors read and approved the final manuscript.

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Correspondence to Ha Danh Duc or Nguyen Thi Oanh.

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Duc, H.D., Oanh, N.T. & Khanh, N.T.M. Thiobencarb Degradation by Pseudomonas sp. Th1 and Cupriavidus oxalaticus Th2 Isolated from Soil. Curr Microbiol 80, 342 (2023). https://doi.org/10.1007/s00284-023-03456-0

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