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The Facile Synthesis of Eco-Friendly Zinc Magnesium Bimetal Nanoparticles and its Application in the Eradication of Xanthomonas oryzae pv. oryzae that Causes Leaf Blight Disease of Rice

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Abstract

In this research work, we formulated and successfully assessed the antibacterial capability of zinc magnesium bimetal nanoparticles (ZnMgNPs) against Xanthomonas oryzae pv. oryzae (Xoo), the pathogenic microorganism responsible for causing the destructive leaf blight disease in rice. Successful preparation of ZnMgNPs were determined by UV-vis spectroscopy, EDX (Energy dispersive X-ray), FTIR (Fourier transform infrared) and SEM (Scanning Electron Microscopy). ZnMgNPs had antibacterial efficacy towards Xoo at MIC (minimum inhibitory concentration) 50 µg/ml. ZnMgNPs impeded the formation of biofilm of Xoo by drastically reducing the amount of EPS (extracellular polymeric substances) production and number of sessile cells. The ZnMgNPs also reduced several pathogenic traits of Xoo like motility, xanthomonadin and exoenzymes production. ZnMgNPs target cell membrane of Xoo and also induced oxidative damage as mechanisms of its antibacterial activity. As revealed by an ex-vivo study, ZnMgNPs diminished BLB (bacterial leaf blight) disease symptoms in rice leaves, ZnMgNPs had no effect on rice seed germination, and that following foliar application, the length and biomass of roots and shoots of rice seedling were unaffected, low cytotoxic to A549 cell line showing that ZnMgNPs are non-toxic. However, with ZnMgNPs treatment, the chlorophyll content index (CCI) increased significantly, indicating a good impact on rice physiology. All of these findings suggest that ZnMgNPs could be applied in agriculture to combat the Xoo-caused BLB disease.

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Data Availability

A copy of the data that support these findings is available on request from the corresponding author.

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Acknowledgements

The authors express their gratitude to the Chancellor of Techno India University, West Bengal, for providing the necessary laboratory facilities and infrastructure. Our heartfelt thanks go to Dr. Bishun Deo Prasad from Bihar Agricultural University, Sabour, Bihar, India, for kindly providing the Xanthomonas oryzae pv. oryzae (Xoo) strain. The authors would also like to acknowledge Mrs. Sheolee Ghosh Chakraborty for her support in utilizing CLSM and Mr. Dipak Chandra Konar for his support with FTIR facilities at Bose Institute, Kolkata, West Bengal, and Mr. Krishanu Paul for his support in accessing SEM-EDS facilities at IIEST, Shibpur, Kolkata.

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Authors and Affiliations

  1. Department of Microbiology, Techno India University, West Bengal EM-4 Sector-V, Saltlake City, Kolkata, West Bengal, 700091, India

    Kumari Vishakha, Shatabdi Das & Arnab Ganguli

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  1. Kumari Vishakha
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  2. Shatabdi Das
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  3. Arnab Ganguli
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Contributions

AG designed the experiments. KV carried out the experiments. AG, KV and SD analysed the data. AG and KV wrote the manuscript. AG supervised the research. All authors carefully read and approved the manuscript.

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Correspondence to Arnab Ganguli.

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Vishakha, K., Das, S. & Ganguli, A. The Facile Synthesis of Eco-Friendly Zinc Magnesium Bimetal Nanoparticles and its Application in the Eradication of Xanthomonas oryzae pv. oryzae that Causes Leaf Blight Disease of Rice. Curr Microbiol 80, 340 (2023). https://doi.org/10.1007/s00284-023-03455-1

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