Abstract
Recent advances fascinated the use of biological resources in combination with metals to obtain high quality biometals and apply its advantages in different fields. Endophytic mediated Zinc oxide nanoparticles (ZnO-NPs) is an economical and ecofriendly way for farmers to avoid Zn deficiency in rice crop and obtain high yield. Here we synthesized ZnO-NPs utilizing endophytic bacterial strain of Enterobacter hormaechei (E. hormaechei). The physiochemical properties of the prepared NPs were determined through UV–Vis spectroscopy, XRD, FT-IR, SEM and TEM. The prepared NPs revealed surface plasmon resonance (SPR) at 320 nm (nm) and crystalline structure with 21 nm average crystalline size. FT-IR spectra showed the presence of carboxylic, alcohol and amine functional groups, which confirm the biometallic assembling of the ZnO and endophytic bacterial functional groups. SEM showed pyramidal symmetry whereas TEM revealed poly dispersed spherical shape with particle size distribution 18–48 nm. Our results showed that prepared NPs possess significant antifungal, antibacterial and antioxidant potential at 25, 50 and 100 µg/mL concentrations. Moreover, Cytotoxic and hemolytic assay showed significant results (less % viability and hemolysis activity) at 50 and 100 µg/mL (ZnO-NP’s) concentrations as compared to control. The prepared ZnO-NPs were used as bio fertilizer in various concentrations as a foliar spray, which showed significant enhancement of the rice plant growth, along with chlorophyll, proteins and carotenoid contents. These results recommend that endophytic mediated ZnO-NPs are biocompatible and possess significant potential for agricultural applications.
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Acknowledgements
We are thankful to department of Biotechnology, Mohi-ud-din Islamic university, Nerian Sahreef, AJ&K, Pakistan and Laboratory of Molecular Systematic and Applied Ethnobotany Lab, Department of Biotechnology, Quaid-i-Azam university, Islamabad, Pakistan for providing basic lab facilities during experiments.
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SS and AN writing original draft. AN, MY and MA methodology and conceptualization. WZ, and SS investigation and formal analysis. MY and MA data curation and supervision. WZ and MY review and editing.
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Saqib, S., Nazeer, A., Ali, M. et al. Catalytic potential of endophytes facilitates synthesis of biometallic zinc oxide nanoparticles for agricultural application. Biometals 35, 967–985 (2022). https://doi.org/10.1007/s10534-022-00417-1
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DOI: https://doi.org/10.1007/s10534-022-00417-1