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Evaluation of Bioremediation Potentiality of Bacillus mojavensis Isolated from Wastewater for the Elimination of Reactive Yellow 145 and Methyl Orange

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Abstract

Textile industry waste has become one of the largest polluters in the world. In recent years, there has been a growing awareness of the need for sustainable and eco-friendly practices for the treatment of dye-laden effluents. Overall, this study highlights the potential of bioremediation as a sustainable solution for wastewater treatment. The Bacillus mojavensis isolated from wastewater and identified using 16S rRNA degraded reactive yellow 145 and methyl orange in 36 h of incubation, this decolorization was affected by pH, temperature, dye concentration, glucose concentration, source of nitrogen, type of dye, and agitation. Our study found that the optimal conditions for total decolorization of dyes were achieved by incubating B. mojavensis at 46 °C, pH 9, with 1 g/L of glucose and 2 g/L of peptone. The azoreductase activity, FT-IR analysis, and UV–visible spectrum before and after total decolorization indicated that it was a dye degradation rather than biosorption in surface Celle. In addition, the study of phytotoxicity show the metabolites of degradation are not phytotoxic in Lens esculenta seeds. In conclusion, our results suggest the use of this bacterium as an environmentally friendly and also cost-effective method, making it an attractive option for industries looking to reduce their environmental impact.

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Authors and Affiliations

  1. Agrofood and Health Laboratory, Faculty of Science and Technology, Hassan First University, PO Box 577, 26000, Settat, Morocco

    Rajaa Sennaj, Khadija Dari & Hinde Aassila

  2. Laboratoire de Biotechnologie et Bioinformatique, École des Hautes Études de Biotechnologie et de Santé (EHEB), Casablanca, Morocco

    Mohammed Timinouni

  3. Environment and Health Team, Polydisciplinary Faculty of Safi, Cadi Ayyad University, B.P. 4162, Sidi Bouzid, Safi, Morocco

    Taoufiq Benali

  4. Université Ibn Zohr Agadir, Agadir, Morocco

    Aziz Fassouane

  5. Natural Ressources and Environment Laboratory, Polydisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University, Fez, Morocco

    Kaoutar Harboul

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  1. Rajaa Sennaj
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Contributions

Conceptualization, R.S., and H.A. methodology; R.S., K.D, M.T, and A.F; validation, R.S., H.A. M.T, and A.F; formal analysis, R.S., T.B., K.H and H.A; writing—original draft preparation, R.S., H.A. M.T, and T.B; supervision, H.A. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Rajaa Sennaj.

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Sennaj, R., Dari, K., Timinouni, M. et al. Evaluation of Bioremediation Potentiality of Bacillus mojavensis Isolated from Wastewater for the Elimination of Reactive Yellow 145 and Methyl Orange. Curr Microbiol 80, 326 (2023). https://doi.org/10.1007/s00284-023-03435-5

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