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Analysis of Endophytic Bacterial Diversity in Rice Seeds with Regional Characteristics in Yunnan Province, China, Based on High-Throughput Sequencing Technology

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Abstract

Examining the endophytic bacteria in rice seeds from Yunnan Province displaying regional characteristics is vital for exploring strain resources, improving rice production, and conducting subsequent research. This study investigated nine characteristic rice varieties from Yunnan Province using high-throughput sequencing technology based on the Illumina Novaseq platform to reveal their dominant bacterial communities and discussed their endophytic bacterial community differences. A total of 829 shared OTUs, and 233 unique OTUs were identified in the nine samples, while the bacteria included Proteobacteria, Actinobacteriota, and Firmicutes, of which Proteobacteria was the most dominant. Pantoea and Methylorubrum were the most abundant at the genus level, with Curtobacterium, Brevundimonas, and Luteibacter representing the specific genera in the rice seed samples. This study revealed the endophytic structure and diversity in the seeds of nine rice varieties displaying regional characteristics and provided a foundation for further research into rice containing endophytic bacteria.

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Data Availability

All data generated or analyzed during this study are included in this published article and its supplementary information files.

Code Availability

Not applicable.

Abbreviations

OTU:

Optical taxonomic unit

PCoA:

Principal coordinates analysis

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Acknowledgements

We would like to thank the research team of Dr. Xupengpeng of Beijing Fixgene Technology Co., Ltd. at Beijing, for its platform and technical support in high-throughput sequencing and bioinformatics analysis.

Funding

The research was supported by the National foreign expert Program of China (No. QN2021105002L), Beijing Nova Program (20220484220), the Science and Technology Talents and Platform of Yunnan province (No. 202105AE160009) and the Fundamental Research Funds for the Central Universities (No. FRF-TP-20-044A2).

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Author notes

  1. Xilong Feng and Zhishan Wang have contributed equally to this work.

Authors and Affiliations

  1. School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China

    Xilong Feng, Zhishan Wang & Yang Liu

  2. Institute of Food Crops Research, Yunnan Academy of Agricultural Sciences, Kunming, 650205, China

    Xiaolin Li & Anyu Gu

  3. Research Center for Wild Animal and Plant Resource Protection and Utilization, Qiongtai Normal University, Haikou, 571127, Hainan, China

    Wei Wang

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  1. Xilong Feng
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  4. Wei Wang
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  5. Anyu Gu
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  6. Yang Liu
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Contributions

XF and ZW: designed and participated in all experimental procedures, performed data analysis, and drafted the manuscript. AG and XL: participated in the plant samples cultivation. WW: was responsible for editing tables and figures. AG: and YL: supervised the study and critically revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wei Wang, Anyu Gu or Yang Liu.

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Feng, X., Wang, Z., Li, X. et al. Analysis of Endophytic Bacterial Diversity in Rice Seeds with Regional Characteristics in Yunnan Province, China, Based on High-Throughput Sequencing Technology. Curr Microbiol 80, 287 (2023). https://doi.org/10.1007/s00284-023-03399-6

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