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Endophytic Fungi as a Promising Source of Anticancer L-Asparaginase: A Review

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Abstract

L-asparaginase is a tetrameric enzyme from the amidohydrolases family, that catalyzes the breakdown of L-asparagine into L-aspartic acid and ammonia. Since its discovery as an anticancer drug, it is used as one of the prime chemotherapeutic agents to treat acute lymphoblastic leukemia. Apart from its use in the biopharmaceutical industry, it is also used to reduce the formation of a carcinogenic substance called acrylamide in fried, baked, and roasted foods. L-asparaginase is derived from many organisms including plants, bacteria, fungi, and actinomycetes. Currently, L-asparaginase preparations from Escherichia coli and Erwinia chrysanthemi are used in the clinical treatment of acute lymphoblastic leukemia. However, they are associated with low yield and immunogenicity problems. At this juncture, endophytic fungi from medicinal plants have gained much attention as they have several advantages over the available bacterial preparations. Many medicinal plants have been screened for L-asparaginase producing endophytic fungi and several studies have reported potent L-asparaginase producing strains. This review provides insights into fungal endophytes from medicinal plants and their significance as probable alternatives for bacterial L-asparaginase.

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Acknowledgements

The authors are thankful to the Council of Scientific and Industrial Research (CSIR) for providing a Junior Research Fellowship (JRF) and DOS in Microbiology, University of Mysore for providing facilities.

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  1. Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysuru, Karnataka, 570 006, India

    Javaraiah Parashiva, Bettadapura Rameshgowda Nuthan & Sreedharamurthy Satish

  2. Department of Microbiology, Yuvaraja’s College, University of Mysore, Manasagangotri, Mysuru, Karnataka, 570 005, India

    Devaraju Rakshith

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Parashiva, J., Nuthan, B.R., Rakshith, D. et al. Endophytic Fungi as a Promising Source of Anticancer L-Asparaginase: A Review. Curr Microbiol 80, 282 (2023). https://doi.org/10.1007/s00284-023-03392-z

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