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Docking Studies on Biomolecules from Marine Microalga Skeletonema costatum Against Hemolysin Protein of Bioluminescence Disease-Causing Vibrio harveyi

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Abstract

Grow-out and hatchery units of shrimps are being impacted by disease-causing bacterial pathogens and predominantly marine Vibrios. The use of chemicals for governing bacterial pathogens in the aquaculture practices is developing resistance to bacteria. Henceforth, the application of bio-therapeutic agents from marine resources for controlling pathogens is most vital to be considered. Molecular docking is computer-assisted drug design tool to detect and counteract for drug–receptor interaction for known target protein of diseases. Therefore, an effort was made with the extract of the marine micro alga Skeletonema costatum against hemolysin protein of pathogenic bacteria Vibrio harveyi. The extract of S. costatum was tested against growth and virulence produced by V. harveyi during larviculture of Penaeus monodon. The extract was analyzed for phyto-constituents through GC–MS and used them as ligand molecule in docking. S. costatum extract at 200 µg mL−1 was found to decrease 35.20% of cumulative percentage mortality (CPM) in postlarvae of P. monodon against V. harveyi infections. The biomolecule Docasane, an alkane from the extract of S. costatum, exposed highest binding interaction than other compounds during docking analysis. The level of significance (P < 0.05) was found in CPM, growth, and virulence factors of V. harveyi studies. Thus, the present finding predicts that extract of S. costatum containing biomolecules can be recommended for use in the shrimp culture-based grow-out and hatchery units for eliminating bioluminescent V. harveyi.

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Acknowledgements

The authors greatly acknowledge the Department of Biotechnology (DBT), New Delhi, India for supporting this work under the sponsored project “Development of inhibitors for controlling quorum sensing luminescence causing Vibrio harveyi in shrimp larviculture system” (BT/PR/13383/AAQ/03/501/2009).

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Authors and Affiliations

  1. ICAR – Krishi Vigyan Kendra, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Kattupakkam, Chennai, Tamil Nadu, 603 203, India

    Krishnamoorthy Sivakumar

  2. Crustacean Culture Division, ICAR – Central Institute of Brackishwater Aquaculture (CIBA), Chennai, Tamil Nadu, 600 028, India

    Sudalayandi Kannappan

  3. Centre of Advance Study in Crystallography and Biophysics, University of Madras, Chennai, Tamil Nadu, 600 025, India

    Balakrishnan Vijayakumar

  4. McArdle Laboratory for Cancer Research, Department of Oncology, School of Medicine and Public Health, University of Wisconsin at Madison, Madison, WI, 53705, USA

    Balakrishnan Vijayakumar

  5. Sao Carlos Institute of Physics (IFSC), University of Sao Paulo, Av. Joao Dagnone, 1100 – Jardim Santa Angelina, Sao Carlos, 13563-120, Brazil

    Balakrishnan Vijayakumar

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KS and SK designed research and wrote the manuscript. KS and BV performed docking work and analyzed data. KS and BV reviewed and edited the manuscript.

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Correspondence to Krishnamoorthy Sivakumar.

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Sivakumar, K., Kannappan, S. & Vijayakumar, B. Docking Studies on Biomolecules from Marine Microalga Skeletonema costatum Against Hemolysin Protein of Bioluminescence Disease-Causing Vibrio harveyi. Curr Microbiol 80, 290 (2023). https://doi.org/10.1007/s00284-023-03372-3

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