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Gut Microbiota in Children with Hand Foot and Mouth Disease on 16S rRNA Gene Sequencing

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Abstract

Hand foot and mouth disease (HFMD) is a contagious and seasonal viral disease in children. The gut microbiota of HFMD children is not clear now. The study aimed to explore the gut microbiota of HFMD children. The 16S rRNA gene of the gut microbiota of ten HFMD patients and ten healthy children were sequenced on the NovaSeq and PacBio platforms respectively. There were significant differences in gut microbiota between the patients and healthy children. The diversity and abundance of gut microbiota in HFMD patients were lower than that in healthy children. The species Roseburia inulinivorans and Romboutsia timonensis were more abundant in healthy children than those in HFMD patients, which suggests that the two species may be used as probiotics for adjusting the gut microbiota of HFMD patients. Meanwhile, the results of 16S rRNA gene sequences from the two platforms were different. The NovaSeq platform identified more microbiota and has the characteristics of high throughput, short time and low price. However, the NovaSeq platform has low resolution at the species level. The PacBio platform has high resolution based on its long reads length, which is more suitable for species-level analysis. But, the shortcomings of the high price and low throughput of PacBio still need to be overcome. With the development of sequencing technology, the reduction in sequencing price and the increase in throughput will promote the third-generation sequencing technology used in the study of gut microbes.

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Data Availability

The 16S rRNA gene sequence data have been deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) database on BioProject accession number PRJNA843173 and PRJNA843181.

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Funding

This work was funded by the Zibo City school city integration development project (2018zbxc215) and the National Natural Science Foundation of China (31870201).

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Author notes

  1. Yan Zhuang and Yiyan Lin are joint first authors.

Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun, 130012, China

    Yan Zhuang, Yiyan Lin & Lu Han

  2. School of Public Health and Laboratory, Qilu Medical University, Zibo, 255300, China

    Yan Zhuang & Rongjun Fan

  3. Department of Paediatric, the First Hospital of Zibo, Zibo, 255200, China

    Hongxia Sun, Zaiting Zhang & Tao Wang

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  1. Yan Zhuang
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Contributions

Conceptualization, RF and LH; methodology, HS, ZZ, and TW; software, YZ and YL; validation, YZ, YL, RF and LH; formal analysis, YZ and YL; investigation, HS, ZZ, and TW; resources, RF and LH; data curation, HS, ZZ, TW, RF and LH; writing—original draft preparation, YZ and YL; writing—review and editing, YZ, YL and LH; visualization, HS, ZZ, TW, YZ and YL; supervision, RF and LH; project administration, RF and LH; funding acquisition, RF and LH. All authors have read and agreed to the published the manuscript.

Corresponding authors

Correspondence to Rongjun Fan or Lu Han.

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The authors declare no conflict of interest.

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The study protocol was approved by the Ethical Committee of the First Hospital of Zibo (LY180003) and was carried out according to a statement from Helsinki.

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Zhuang, Y., Lin, Y., Sun, H. et al. Gut Microbiota in Children with Hand Foot and Mouth Disease on 16S rRNA Gene Sequencing. Curr Microbiol 80, 159 (2023). https://doi.org/10.1007/s00284-023-03277-1

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