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Limnobacter parvus sp. nov., a Thiosulfate-Oxidizing Bacterium Isolated from Lake Water

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Abstract

A novel bacterium, designated as strain YS8-69T, was isolated from an inland closed lake, Xinjiang Uygur Autonomous Region, PR China. Comparative analysis of the 16S rRNA gene sequence shows the strain was affiliated to the genus Limnobacter, in the family Burkholderiaceae, with the highest similarities to Limnobacter alexandrii LZ-4T (98.93%), Limnobacter thiooxidans DSM 13612T (98.55%), Limnobacter humi NBRC 111650T (97.66%), and Limnobacter litoralis KP1-19T (97.04%). Strain YS8-69T was a Gram stain-negative, strictly aerobic, rod shaped, catalase- and oxidase-positive bacterium, and growth was observed at 4–40 °C (optimum, 25 °C), pH 7.0–10.0 (optimum, pH 7.0), and 0–3% (w/v) NaCl (optimum, 0.5%). The principal fatty acids were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The sole respiratory quinone was Q-8 and total polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unidentified aminolipid (AL), two unidentified glycolipids (GL1,2), an unidentified amino phosphoglycolipid (APGL), two unidentified phospholipids (PL1,2), two unidentified aminophospholipids (APL1,2), and three unidentified lipids (L1,2,3). The average nucleotide identity (ANI) values and in silico DDH between strain YS8-69T and L. alexandrii LZ-4T, L. thiooxidans JCM 13612T, and L. humi DSM 111650T were 73.0–80.6% and 15.8–50.2%, respectively. The genome sequence showed a length of 3,162,663 bp, with 20 contigs and 51.7% of G + C content. Based on physiological, chemotaxonomic, genotypic characteristics, and phylogenetic results, we propose that strain YS8-69T represents a novel specie of the genus Limnobacter, for which the name Limnobacter parvus sp. nov. is proposed (type strain YS8-69T = MCCC 1K08015T = KCTC 92278T).

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Data Availability

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence and whole-genome sequence of strain YS8-69T is OM462841 and JANKHG000000000, respectively. The whole-genome sequence project of Limnobacter humi NBRC 111650T has been deposited under the accession JANIGO000000000.

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Acknowledgements

We are grateful to You-ping Xu (Zhejiang University Analysis Center of Agrobiology and Environmental Sciences) for helping to analyze the fatty acid composition.

Funding

This work was supported by the Key R&D Program of Zhejiang (2022C03010) and the Science and Technology Basic Resources Investigation Program of China (2017FY100300).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Maripat Xamxidin, Xinpeng Huang, Xiangwei Yang, Can Chen & Min Wu

  2. Department of Microbiology, University of Georgia, Athens, GA, USA

    Tao Wang

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  1. Maripat Xamxidin
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  4. Tao Wang
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Contributions

MW and CC designed the experiments and guided the manuscript writing. MX was responsible for the major experiments, data analysis, and preparation of manuscripts. XPH and XWY assisted in enzymatic experiments and determination of polar lipids experiment. TW revised the manuscript. All authors read and approved the manuscript.

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Correspondence to Can Chen or Min Wu.

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Xamxidin, M., Huang, X., Yang, X. et al. Limnobacter parvus sp. nov., a Thiosulfate-Oxidizing Bacterium Isolated from Lake Water. Curr Microbiol 80, 39 (2023). https://doi.org/10.1007/s00284-022-03128-5

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