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Effect of Berberine and Blue LED Irradiation on Combating Biofilm of Pseudomonas aeruginosa and Staphylococcus aureus

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Abstract

Nowadays, with increasing resistance of microorganisms to drugs, it is necessary to look for new solutions beside antibiotic therapy. One of these effective approaches is the use of plant compounds and blue LED Irradiation. Berberine (an alkaloid compound) has several properties, including antibacterial effect. This compound destroys bacterial cells by producing reactive oxygen species (ROS). In this study, the combined effect of blue LED Irradiation and berberine on Pseudomonas aeruginosa (Gram-negatives) and Staphylococcus aureus (Gram-positive) and also their effect on human dermal fibroblast (HDF) cells were investigated. The obtained results showed that the combination of berberine and blue light irradiation had a better effect on both bacteria and this antimicrobial effect was higher in Gram-positive bacteria. These compounds also prevented the formation of biofilms and were able to destroy the created biofilms. Therefore, this method can be suggested to treat infection in chronic wounds, such as diabetic wounds.

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References

  1. Motallebi M, Khorsandi K, Sepahy AA, Chamani E, Hosseinzadeh R (2020) Effect of rutin as flavonoid compound on photodynamic inactivation against P. aeruginosa and S. aureus. Photodiagn Photodyn Ther 32:102074. https://doi.org/10.1016/j.pdpdt.2020.102074

    Article  CAS  Google Scholar 

  2. Alaghehbandan R, Azimi L, Rastegar Lari A (2012) Nosocomial infections among burn patients in Teheran, Iran A decade later. Ann Burns Fire Disasters 25(1):3–7

    CAS  PubMed  PubMed Central  Google Scholar 

  3. Cornelis P (2020) Putting an end to the Pseudomonas aeruginosa IQS controversy. Microbiologyopen 9(2):2–3. https://doi.org/10.1002/mbo3.962

    Article  Google Scholar 

  4. Streeter K, Katouli M (2016) Pseudomonas aeruginosa: a review of their pathogenesis and prevalence in clinical settings and the environment. Infect Epidemiol Med 2(1):25–32. https://doi.org/10.18869/modares.iem.2.1.25

    Article  Google Scholar 

  5. Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG (2015) Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 28(3):603–661. https://doi.org/10.1128/CMR.00134-14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Nimer NA (2022) Nosocomial infection and antibiotic-resistant threat in the middle east. Infect Drug Resist 15:631–639. https://doi.org/10.2147/IDR.S351755

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. López D, Vlamakis H, Kolter R (2010) Biofilms. Cold Spring Harb Perspect Biol. https://doi.org/10.1101/cshperspect.a000398

    Article  PubMed  PubMed Central  Google Scholar 

  8. She P et al (2018) Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa. MicrobiologyOpen. https://doi.org/10.1002/mbo3.545

    Article  PubMed  PubMed Central  Google Scholar 

  9. Mirzahosseinipour M, Khorsandi K, Hosseinzadeh R, Ghazaeian M, Shahidi FK (2020) Antimicrobial photodynamic and wound healing activity of curcumin encapsulated in silica nanoparticles. Photodiagnosis Photodyn Ther 29:101639. https://doi.org/10.1016/j.pdpdt.2019.101639

    Article  CAS  PubMed  Google Scholar 

  10. Wang Y et al (2016) Antimicrobial blue light inactivation of Gram-negative pathogens in biofilms. In vitro and in vivo studies. J Infect Dis 213(9):1380–1387. https://doi.org/10.1093/infdis/jiw070

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Bush K et al (2011) Tackling antibiotic resistance. Nat Rev Microbiol 9(12):894–896. https://doi.org/10.1038/nrmicro2693

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Dai T, Gupta A, Murray CK, Vrahas MS, Tegos GP, Hamblin MR (2012) Blue light for infectious diseases: Propionibacterium acnes, Helicobacter pylori, and beyond? Drug Resist Updates 15(4):223–236. https://doi.org/10.1016/j.drup.2012.07.001

    Article  Google Scholar 

  13. Hamblin MR, Abrahamse H (2019) Can light-based approaches overcome antimicrobial resistance? Drug Dev Res 80(1):48–67. https://doi.org/10.1002/ddr.21453

    Article  CAS  PubMed  Google Scholar 

  14. Kleinpenning MM, Smits T, Frunt MHA, van Erp PEJ, van de Kerkhof PCM, Gerritsen RMJP (2010) Clinical and histological effects of blue light on normal skin. Photodermatol Photoimmunol Photomed 26(1):16–21. https://doi.org/10.1111/j.1600-0781.2009.00474.x

    Article  PubMed  Google Scholar 

  15. Liebmann J, Born M, Kolb-Bachofen V (2010) Blue-light irradiation regulates proliferation and differentiation in human skin cells. J Investig Dermatol 130(1):259–269. https://doi.org/10.1038/jid.2009.194

    Article  CAS  PubMed  Google Scholar 

  16. Masson-Meyers DS, Bumah VV, Enwemeka CS (2016) Blue light does not impair wound healing in vitro. J Photochem Photobiol, B 160:53–60. https://doi.org/10.1016/j.jphotobiol.2016.04.007

    Article  CAS  Google Scholar 

  17. Mahmoudi H, Fahim NZ, Alikhani MY, Shokoohizadeh L (2020) Investigation of antimicrobial effect of berberine on ciprofloxacin and imipenem resistance Acinetobacter baumannii Isolated from Hamadan hospitals. Iran J Med Microbiol 14(1):44–54. https://doi.org/10.30699/ijmm.14.1.44

    Article  Google Scholar 

  18. Jung J, Park W (2015) Acinetobacter species as model microorganisms in environmental microbiology: current state and perspectives. Appl Microbiol Biotechnol 99(6):2533–2548. https://doi.org/10.1007/s00253-015-6439-y

    Article  CAS  PubMed  Google Scholar 

  19. I. F. Hall, First Italian-Lebanese Science Day “Global Science ” The Lebanese Association for the Advancement of Science The American University of Beirut The University of Palermo , Italy The National Council for Scientific Research-Lebanon American University of B, no. First Italian-Lebanese Science Day “Global Science.” 2019.

  20. Laudadio E et al (2019) Natural alkaloid berberine activity against Pseudomonas aeruginosa MexXY-mediated aminoglycoside resistance. In silico and in vitro studies. J Nat Prod 82(7):1935–1944. https://doi.org/10.1021/acs.jnatprod.9b00317

    Article  CAS  PubMed  Google Scholar 

  21. Wang X et al (2009) Effect of berberine on Staphylococcus epidermidis biofilm formation. Int J Antimicrob Agents 34(1):60–66. https://doi.org/10.1016/j.ijantimicag.2008.10.033

    Article  CAS  PubMed  Google Scholar 

  22. Das S et al (2020) Isolation, characterization of Berberine from Berberis aristata DC for eradication of resistant Helicobacter pylori. Biocatalysis Agric Biotechnol 26:101622. https://doi.org/10.1016/j.bcab.2020.101622

    Article  Google Scholar 

  23. Peng L, Kang S, Yin Z, Jia R, Song X, Li L, Li Z, Zou Y, Liang X, Li L, He C (2015) Lianci peng, “antibacterial activity and mechanism of berberine against Streptococcus agalactiae”. Int J Clin Exp Pathol 8(5):5217–5223

    PubMed  PubMed Central  Google Scholar 

  24. Zhang X et al (2020) Berberine damages the cell surface of methicillin-resistant Staphylococcus aureus. Fron Microbiol. https://doi.org/10.3389/fmicb.2020.00621

    Article  Google Scholar 

  25. Xia S et al (2022) In vitro antimicrobial activity and the mechanism of berberine against methicillin-resistant Staphylococcus aureus isolated from bloodstream infection patients. Infect Drug Resistance 15:1933–1944. https://doi.org/10.2147/IDR.S357077

    Article  Google Scholar 

  26. Sun D, Courtney HS, Beachey EH (1988) Berberine sulfate blocks adherence of Streptococcus pyogenes to epithelial cells, fibronectin, and hexadecane. Antimicrob Agents Chemother 32(9):1370–1374. https://doi.org/10.1128/AAC.32.9.1370

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Cieplik F et al (2018) Antimicrobial photodynamic therapy–what we know and what we don’t. Crit Rev Microbiol 44(5):571–589. https://doi.org/10.1080/1040841X.2018.1467876

    Article  CAS  PubMed  Google Scholar 

  28. Plavskii VY et al (2018) Porphyrins and flavins as endogenous acceptors of optical radiation of blue spectral region determining photoinactivation of microbial cells. J Photochem Photobiol, B 183(April):172–183. https://doi.org/10.1016/j.jphotobiol.2018.04.021

    Article  CAS  Google Scholar 

  29. Murdoch LE, MacLean M, Endarko E, MacGregor SJ, Anderson JG (2012) Bactericidal effects of 405nm light exposure demonstrated by inactivation of escherichia, salmonella, Shigella, Listeria, and mycobacterium species in liquid suspensions and on exposed surfaces. Sci World J. https://doi.org/10.1100/2012/137805

    Article  Google Scholar 

  30. Lauro FM, Pretto P, Covolo L, Jori G, Bertoloni G (2002) Photoinactivation of bacterial strains involved in periodontal diseases sensitized by porphycene–polylysine conjugates. Photochem Photobiol Sci 1(7):468–470. https://doi.org/10.1039/b200977c

    Article  CAS  PubMed  Google Scholar 

  31. Moustafa Y, Kassab AN, el Sharnoubi J, Yehia H (2013) Comparative study in the management of allergic rhinitis in children using LED phototherapy and laser acupuncture. Int J Pediatr Otorhinolaryngol 77(5):658–665. https://doi.org/10.1016/j.ijporl.2013.01.006

    Article  PubMed  Google Scholar 

  32. Volpato LER, de Oliveira RC, Espinosa MM, Bagnato VS, Machado MAAM (2011) Viability of fibroblasts cultured under nutritional stress irradiated with red laser, infrared laser, and red light-emitting diode. J Biomed Opt 16(7):075004. https://doi.org/10.1117/1.3602850

    Article  CAS  PubMed  Google Scholar 

  33. White A, Hughes JM (2019) Critical importance of a one health approach to antimicrobial resistance. EcoHealth 16(3):404–409. https://doi.org/10.1007/s10393-019-01415-5

    Article  PubMed  Google Scholar 

  34. Liu B, Wang G, Yang J, Pan X, Yang Z, Zang L (2011) Berberine inhibits human hepatoma cell invasion without cytotoxicity in healthy hepatocytes. PLoS ONE 6(6):e21416. https://doi.org/10.1371/journal.pone.0021416

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. el khalki L, Tilaoui M, Jaafari A, Ait Mouse H, Zyad A (2018) Studies on the dual cytotoxicity and antioxidant properties of Berberis vulgaris extracts and its main constituent berberine. Adv Pharmacol Sci. https://doi.org/10.1155/2018/3018498

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge all the people who helped us to do this project.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Biology Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Sadaf Mahuti Safai

  2. Department of Photodynamic, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran

    Khatereh Khorsandi

  3. Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, The George Washington University, Washington, DC, 20037, USA

    Khatereh Khorsandi

  4. Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Science, Islamic Azad University, Tehran, Iran

    Sarvenaz Falsafi

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  1. Sadaf Mahuti Safai
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Contributions

SMS performed the experiments. KK designed and supervised the experiments. SMS and KK contributed to the data collection and draft the manuscript. KK read and edited the manuscript. KK and SF edited the final version. All authors gave final approval.

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Correspondence to Khatereh Khorsandi.

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Safai, S.M., Khorsandi, K. & Falsafi, S. Effect of Berberine and Blue LED Irradiation on Combating Biofilm of Pseudomonas aeruginosa and Staphylococcus aureus. Curr Microbiol 79, 366 (2022). https://doi.org/10.1007/s00284-022-03063-5

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