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Novel Probiotic Lactic Acid Bacteria with In Vitro Bioremediation Potential of Toxic Lead and Cadmium

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Abstract

In this study, newly isolated lactic acid bacteria (LAB) were screened for the potential bioremediation capacity against toxic lead (Pb, II) and cadmium (Cd, II) with their bioaccessibility and survivability. Five strains were selected from eighteen previously isolated probiotic LAB strains based on heavy metal-resistant potentiality through in vitro disc-diffusion assay. These five strains were evaluated in vitro to explore the Pb and Cd binding and removal efficiencies using Flame Atomic Absorption Spectrophotometry. At the same time, their bioaccessibility and survivability were assessed in a dynamic in vitro gastrointestinal digestion model. The results revealed that all the tested strains were shown to have a high magnitude of minimum inhibitory concentration values ranging from 500 to 2000 mg/L with 5 to 25 mm growth inhibition zones. The results also demonstrated a significant (P < 0.05) removal of Pb and Cd among five tested LAB strains. Lactobacillus delbrueckii subsp. bulgaricus LDMB02 showed the highest removal rates of Pb and Cd. It was also revealed that these strains significantly reduced Pb and Cd bioaccessibility from 42 to 50% and 40 to 58%, respectively. Moreover, these strains were shown to have significant survivability against Pb and Cd, ranging from 80.1 to 85.4% and 81.5 to 87.5%, respectively. This study recommends the immense potential exploit of LAB as a probiotic to protect human health from the adverse effects of Pb and Cd toxicity.

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Data and material of this research are accessible from the corresponding authors upon rational request.

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Acknowledgements

The authors acknowledge the Ministry of Science and Technology for their financial support. The authors also thank the Dairy Microbiology and Biotechnology Lab, Department of Dairy Science, and Food Science Lab, Interdisciplinary Institute for Food Security, Bangladesh Agricultural University, Mymensingh, for their necessary facilities.

Funding

This work was supported by National Science and Technology (NST) fellowship (298, 2019-2020), Ministry of Science and Technology, Dhaka, Bangladesh.

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  1. Md. Sayed Hasan and Md. Zakirul Islam have contributed equally to this work.

Authors and Affiliations

  1. Department of Dairy Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Md. Sayed Hasan, Md. Zakirul Islam, Md. Abid Hasan Sarker, Mohammad Ashiqul Islam & Md. Harun-ur-Rashid

  2. Department of Animal Nutrition, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh

    Ruckshana Islam Liza

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Contributions

MSH: Formal investigation, analysis, writing first & original draft, review & edited final manuscript. MZI: Conceptualization & study design, set-up & optimized biomimetic digestion model, formal investigation, data curation & analysis, graphical presentation, writing original draft and review & edited final manuscript. RIL: Review and manuscript proofreading. MAHS: Review & edited the draft & final manuscript. MAI: Supervision, technical support, review & edited final manuscript. MHR: Supervision and allocation of lab resources for this research work.

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Correspondence to Md. Zakirul Islam or Md. Harun-ur-Rashid.

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284_2022_3059_MOESM1_ESM.tiff

Supplementary file1 (TIFF 14301 kb). Figure S1. Growth inhibition zones at different Pb and Cd concentrations on MRS agar media (a) Lactobacillus acidophilus LDMB01; (b) Lactobacillus delbrueckii subsp. bulgaricus LDMB02; (c) Lacticaseibacillus casei subsp. casei LDMB03; (d) Pediococcus pentosaceus LDMB08; and (e) Lactococcus lactis LDMB10. A, B and C indicate the heavy metal concentrations at 50, 250, and 2000 mg/L, respectively. The plates are labeled with Pb = Lead and Cd = Cadmium, and the disc without labeling indicates the control group

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Hasan, M.S., Islam, M.Z., Liza, R.I. et al. Novel Probiotic Lactic Acid Bacteria with In Vitro Bioremediation Potential of Toxic Lead and Cadmium. Curr Microbiol 79, 387 (2022). https://doi.org/10.1007/s00284-022-03059-1

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