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Analysis of Blueberry Plant Rhizosphere Bacterial Diversity and Selection of Plant Growth Promoting Rhizobacteria

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Abstract

Microbial metabolites in rhizosphere soil are important to plant growth. In this study, microbial diversity in blueberry plant rhizosphere soil was characterized using high-throughput amplicon sequencing technology. There were 11 bacterial phyla and three fungal phyla dominating in the soil. In addition, inorganic-phosphate-solubilizing bacteria (iPSB) in the rhizosphere soil were isolated and evaluated by molybdenum-antimony anti-coloration method. Their silicate solubilizing, auxin production, and nitrogen fixation capabilities were also determined. Eighteen iPSB in the rhizosphere soil strains were isolated and identified as Buttiauxella, Paraburkholderia and Pseudomonas. The higher phosphorus-solubilizing capacity and auxin production in blueberry rhizosphere belonged to genus Buttiauxella sp. The strains belong to genus Paraburkholderia had the same function of dissolving both phosphorus and producing auxin, as well as silicate and nitrogen fixation. The blueberry seeds incubated with the strains had higher germination rates. The results of this study could be helpful in developing the plant growth-promoting rhizobacteria (PGPR) method for enhancing soil nutrients to blueberry plant.

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Funding

This study was supported by the Foundation of Young Scientist Empowerment Project of Shaanxi Association of Science and Technology (20200206), Natural Science Basic Research Program of Shaanxi (2021JQ-755), and the Foundation of Shaanxi University of Technology (SLG1809), China.

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Authors and Affiliations

  1. School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, China

    Mengjiao Wang & Haiyan Sun

  2. Shaanxi Key Laboratory of Bioresources, Shaanxi University of Technology, Hanzhong, Shaanxi, China

    Haiyan Sun

  3. School of Nutrition and Food Sciences, Louisiana State University, Baton Rouge, LA, USA

    Zhimin Xu

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  1. Mengjiao Wang
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  3. Zhimin Xu
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by MW, HS and ZX. The first draft of the manuscript was written by MW and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.”

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Correspondence to Mengjiao Wang.

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Appendix

Appendix

See Figs. 6 and 7

Fig. 6
figure 6

Alpha diversity of microorganism in the rhizosphere soil of studied blueberry species. A Shannon indices of bacteria in soil samples; B Simpson indices of bacteria in soil samples; C Shannon indices of fungus in soil samples; D Simpson indices of fungus in soil samples

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Fig. 7
figure 7

Typical phosphorus decomposition halos by iPSB strains isolated from blueberry plant rhizosphere soils

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Wang, M., Sun, H. & Xu, Z. Analysis of Blueberry Plant Rhizosphere Bacterial Diversity and Selection of Plant Growth Promoting Rhizobacteria. Curr Microbiol 79, 331 (2022). https://doi.org/10.1007/s00284-022-03031-z

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