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Production, Partial Purification, and Characterization of Polygalacturonase from Aureobasidium pullulans P56 under Submerged Fermentation Using Agro-Industrial Wastes

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Abstract

Polygalacturonase (PGase) production by Aureobasidium pullulans P56 under submerged fermentation was investigated using agro-industrial wastes and commercial carbon and nitrogen sources. The maximum PGase concentration was equivalent to 8.6 U/mL that was obtained in presence of citrus pectin at 150 rpm, 30 °C, pH = 5.5, and 60 h of fermentation conditions. However, a significant amount of enzyme production was also recorded upon the utilization of corncob (5.3 U/mL) and wheat bran (4.4 U/mL) as carbon sources. Amongst the different nitrogen sources, the highest enzyme production (8.2 U/mL) was obtained in presence of ammonium sulphate and yeast extract simultaneously at a ratio of 1:1. The enzyme was partially purified by gel filtration using Sephadex G50 equilibrated and washed with 50 mM-sodium acetate buffer. The obtained yield and specific activity were determined equivalent to 17% and 9.53 U/mg, respectively. The molecular weight of the partially purified enzyme was estimated as 54 kDa on SDS-PAGE. The conditions affecting the enzyme activity were determined and the highest enzyme activity was recorded at 40 °C and 4.5 pH. Amongst the tested metal ions, 2 and 5 mM of CaCl2 concentrations increased the enzymatic activity by 30%. Overall, the use of corncob (2.5%) to produce PGase by A. pullulans represents an attractive agro-industrial substrate.

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Data Availability

The data used to support the findings of this study are available upon reasonable request. A. pullulans p56 has been deposited at the Culture Collection of the Biology Department of the Manisa Celal Bayar University, Turkey with an accession number CCB 012.

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Not applicable.

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Acknowledgements

I would like to thank the Manisa Celal Bayar University for Partial financial support.

Funding

Partial financial support was received from Manisa Celal Bayar University under project number 2015–093.

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  1. Faculty of Sciences and Letters, Department of Biology, Section of Basic and Industrial Microbiology, Manisa Celal Bayar University, 45030, Manisa, Turkey

    Mustafa Oskay

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Oskay, M. Production, Partial Purification, and Characterization of Polygalacturonase from Aureobasidium pullulans P56 under Submerged Fermentation Using Agro-Industrial Wastes. Curr Microbiol 79, 296 (2022). https://doi.org/10.1007/s00284-022-02991-6

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