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Chitosan and Gold Nanoparticles Supplementation for Augmentation of Indole-3-Acetic Acid Production by Rhizospheric Pseudomonas aeruginosa and Plant Growth Enhancement

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Abstract

The present study has been focused to evaluate the effect of chitosan nanoparticles (CNPs) and gold nanoparticles (AuNPs) on the phytohormone production by rhizospheric Pseudomonas aeruginosa. The gold nanoparticles were synthesized biologically and characterized by UV–Visible spectrophotometry, transmission electron microscopy, and X-ray diffraction analysis. The indole-3-acetic acid (IAA) production by P. aeruginosa supplemented with CNPs and AuNPs was quantified by using Salkowski’s method and confirmed by high-performance liquid chromatography (HPLC) analysis. This revealed the effect of 5 mg/mL CNPs and 100 µg/mL AuNPs to enhance the IAA production by P. aeruginosa. By Salkowski’s method, 07.16 ± 0.28 and 09.56 ± 0.28 µg/mL of IAA could be detected in the samples prepared from P. aeruginosa supplemented with 5 mg/mL CNPs and 100 µg/mL AuNPs, respectively. HPLC analysis also confirmed the production of IAA by P. aeruginosa. The CNPs and AuNPs-supplemented P. aeruginosa was also found to have enhancement effect on the shoot length (25.25 ± 0.85 cm and 26.57 ± 0.73 cm) and fresh weight (0.94 ± 0.09 g and 0.96 ± 0.09 g) of Vigna unguiculata plants, which highlight the significance of the study and the agricultural promises of nanomaterials-supplemented rhizobacteria.

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Acknowledgements

The authors acknowledge Kerala State Council for Science, Technology and Environment (KSCSTE) under the KSCSTE-SRS Scheme for financial support and KSCSTE-KBC-YIPB, Kerala State Plan Fund Project, Jaivam Project and Department of Biotechnology (DBT-MSUB), School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India, for instrumentation facility. The authors also acknowledge the Director, Inter-University Instrumentation Centre, Mahatma Gandhi University, Kottayam, for providing HPLC analysis facility.

Funding

The study was financially supported by Kerala State Council for Science, Technology and Environment (KSCSTE) under the KSCSTE-SRS Scheme.

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Authors and Affiliations

  1. School of Biosciences, Mahatma Gandhi University, PD Hills (PO),, Kottayam, Kerala, 686 560, India

    Jishma Panichikkal & Radhakrishnan E. Krishnankutty

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  1. Jishma Panichikkal
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  2. Radhakrishnan E. Krishnankutty
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Contributions

REK contributed to the study conception and design. Experiments and analysis were performed by JP. The first draft of the manuscript was written by JP. The manuscript was revised and corrections were included by REK. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Radhakrishnan E. Krishnankutty.

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Jishma Panichikkal and Radhakrishnan E. Krishnankutty declare that they have no conflict of interest.

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Panichikkal, J., Krishnankutty, R.E. Chitosan and Gold Nanoparticles Supplementation for Augmentation of Indole-3-Acetic Acid Production by Rhizospheric Pseudomonas aeruginosa and Plant Growth Enhancement. Curr Microbiol 79, 185 (2022). https://doi.org/10.1007/s00284-022-02850-4

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