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Rhodobacter kunshanensis sp. nov., a Novel Bacterium Isolated from Activated Sludge

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Abstract

Strain HX-7-19T was isolated from the activated sludge collected from an abandoned herbicide manufacturing plant in Kunshan, China. Cells were Gram-reaction-negative, rod-shaped, and non-motile. The phylogenetic analysis based on 16S rRNA gene indicated that strain HX-7-19T formed a clade with Rhodobacter blasticus CGMCC 1.3365T (96.3% sequence similarity). The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain HX-7-19T and R. blasticus CGMCC 1.3365T were 76.2% and 20.3%, respectively. The genomic DNA G + C content of strain HX-7-19T was 65.9%. The major fatty acids (> 10% of the total fatty acids) were C18:1 ω7c and C18:1 ω7c 11-methyl. The major respiratory quinone was quinone Q-10. The major polar lipid profile consists of phosphatidylglycerol (PG), diphosphatidyl-glycerol (DPG), phosphatidylethanolamine (PE), and phosphatidylcholine (PC). Photosynthesis pigments bacteriochlorophyll a and carotenoids were formed and photosynthesis genes pufL and pufM were detected. On the basis of phenotypic and phylogenetic evidences, strain HX-7-19T is considered as a novel species in the genus Rhodobacter, for which the name Rhodobacter kunshanensis sp. nov. is proposed. The type strain is HX-7-19T (= KCTC 72471T = CCTCC AB 2020148T).

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Acknowledgements

This study was funded by the National Natural Science Foundation of China (Grant Number 32070092).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People’s Republic of China

    Junwei Liu, Yixuan Bao, Xuan Zhang, Siqiong Xu, Jiguo Qiu & Jian He

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  1. Junwei Liu
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  2. Yixuan Bao
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  3. Xuan Zhang
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  4. Siqiong Xu
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  5. Jiguo Qiu
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  6. Jian He
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Contributions

JQ, JL, and JH carried out the concepts. JL and YB participated in the research and analyzed the data. XZ and SX provided assistances for literature search and data acquisition. JL drafted the manuscript. JQ and JH performed manuscript review. All authors read and approved the final manuscript.

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Correspondence to Jiguo Qiu.

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The research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. All the authors declare that they have no conflict of interest.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences and the whole genome of strains HX-7-19T are MT101853 and JAALFE000000000, respectively.

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Liu, J., Bao, Y., Zhang, X. et al. Rhodobacter kunshanensis sp. nov., a Novel Bacterium Isolated from Activated Sludge. Curr Microbiol 78, 3791–3797 (2021). https://doi.org/10.1007/s00284-021-02628-0

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