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Bacterial Diversity and Community Structure in the Rhizosphere of Four Halophytes

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Abstract

The study of the rhizosphere microbial community in salinized soils aids in the elucidation of new and important microbial functional groups, which is of great importance in vegetation restoration and ecological reconstruction of salinized soil. The rhizosphere soil bacterial diversity and community structures of four halophytes, including Kalidium foliatum, Lycium ruthenicum, Karelinia caspia and Phragmites australis, typically distributed in the saline-alkaline land of Southern Xinjiang, China, were studied using an Illumina paired-end sequence platform. The study aims to reveal the alpha diversity, species composition, abundance and the differences of rhizosphere bacteria among the four halophytes, explore their correlation with environmental factors. The results showed that the highest bacterial species diversity was associated with P. communis, followed by K. foliatum, K. caspia, and L. ruthenicum. The species richness was the lowest for L. ruthenicum, while the others showed no significant difference. Firmicutes, Proteobacteria, Actinobacteria and Bacteroidetes were the most dominant phyla. And Bacillus and Halomonas were the most common dominant genera. The bacterial communities associated with K. foliatum and K. caspia were similar, while that of L. ruthenicum was significantly different from other halophytes. Soil total nitrogen and total phosphorus, soil organic matter, soil water content, electronic conductivity and pH were identified as the key factors affecting bacterial abundance associated with the assayed halophytes. These results indicate that the four halophytes evaluated in the present study have a similar rhizosphere bacterial community structure due to their being in the same region. However, the bacterial abundance is different among the plant species, and soil properties are the important factors driving the structures of bacterial communities.

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Acknowledgements

This study was supported by grants from the Scientific Research Program of the Higher Education Institution of XinJiang, China (Grant No. XJEDU2018Y038) and the National Key Research and Development Project of China (Grant No. 2019YFC0507703).

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Authors and Affiliations

  1. College of Grassland Science, Gansu Agricultural University, Lanzhou, 730000, China

    Mingyuan Li & Tuo Yao

  2. College of Biologic and Geographic Sciences, Kashi University, Kashi, 844000, China

    Mingyuan Li, Jilian Wang, Tian Zhang, Qian Zhou &  Mihray·Mutallip

  3. Key Laboratory of Ecology and Biological Resources in Yarkand Oasis of Education of Xinjiang Uygur Autonomous Region, Kashi, 844000, China

    Mingyuan Li, Jilian Wang, Tian Zhang, Qian Zhou &  Mihray·Mutallip

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Contributions

ML and TY designed the experiment, reviewed and edited the manuscript. TZ, QZ and MM carried out the field sampling, JW took part in data analysis and the writing of the manuscript.

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Correspondence to Tuo Yao.

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Li, M., Wang, J., Yao, T. et al. Bacterial Diversity and Community Structure in the Rhizosphere of Four Halophytes. Curr Microbiol 78, 2720–2732 (2021). https://doi.org/10.1007/s00284-021-02536-3

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