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Cloning and Characterization of phzR Gene from Pseudomonas aeruginosa

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Abstract

A gene encoding phzR was isolated from a phenazine-producing bacterium Pseudomonas aeruginosa 2016NX1. This paper provided the full-length cDNA encoding phzR (GenBank Accession no., MW143078). The cDNA of the phzR contained an open reading frame (ORF) of 714 bp. The potential regulatory elements were predicted in the phzR promoter region. The deduced amino acid sequence of P. aeruginosa phzR showed significant homology to the known phzRs from different organisms. Gene overexpression analysis showed that the phenazine content was improved (44.39%) in comparison to wild-type 2016NX1.

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Acknowledgements

This research was funded by National Natural Science Foundation of China (No. 31860010), Natural Science Foundation of Guangxi Zhuang autonomous region (No. 2017JJA130300y and 2018GXNSFAA138008), Science and Technology Program of Guangzhou, China (No. 201804010155), Scientific Research Project of Guangxi Normal University (No. 2019YR004) and Ecological Doctoral Program Construction of Guangxi Normal University (No. EDPC 2018001).

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Authors and Affiliations

  1. College of Life Science, Guangxi Normal University, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China

    Bingbing Pang, Tingting Liu, Wenjia Zhang, Fengcai Ye & Changhua Shang

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  1. Bingbing Pang
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  2. Tingting Liu
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  3. Wenjia Zhang
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  4. Fengcai Ye
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  5. Changhua Shang
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Contributions

Bingbing Pang and Tingting Liu wrote the main part of the paper and performed the experiments and contributed equally to this work. Wenjia Zhang and Fengcai Ye wrote the less part of the paper. Changhua Shang conceived the experiments and proofread the paper. All authors read and approved the manuscript.

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Correspondence to Changhua Shang.

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The study has been conducted under the national regulations. The name of the body is given ethical approval.

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Pang, B., Liu, T., Zhang, W. et al. Cloning and Characterization of phzR Gene from Pseudomonas aeruginosa. Curr Microbiol 78, 1482–1487 (2021). https://doi.org/10.1007/s00284-021-02410-2

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  • DOI: https://doi.org/10.1007/s00284-021-02410-2

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