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Genetic determinants involved in the biodegradation of naphthalene and phenanthrene in Pseudomonas aeruginosa PAO1

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Abstract

Pseudomonas sp. are predominant isolates of degradation-competent strains while very few studies have explored the degradation-related genes and pathways in most of the degrading strains. P. aeruginosa PAO1 was found capable of degrading naphthalene and phenanthrene efficiently. In order to investigate the degradation-related genes of naphthalene and phenanthrene in P. aeruginosa PAO1, a random promoter library of about 5760 strains was constructed. Thirty-two clones for differentially expressed promoters were obtained by screening in the presence of sub-inhibitory concentration of naphthalene and phenanthrene. Among them, 13 genes were up-regulated and 15 were down-regulated in the presence of naphthalene as well as phenanthrene. The four remaining genes have different regulation tendencies by naphthalene or phenanthrene. By comparing the growth between the wild type and mutants as well as the complementations, the roles of seven selected up-regulated genes on naphthalene and phenanthrene degradation were investigated. Five of the seven selected up-regulated genes, like PA2666 and PA4780, were found playing key roles on the degradation in P. aeruginosa PAO1. Also, the results imply that these genes participate in the overlapping part of naphthalene and phenanthrene degradation pathways in PAO1. Results in the article offer the convenience quick method and platform for searching degradation-related genes. It also laid a foundation for understanding of the role of the regulated genes.

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Acknowledgments

This work was supported by NSFC (Grant No: 81171620, 1210063), PCSIRT (No. IRT1174), Shaanxi Provincial Government (No. 11JK0611) and the Western Resource Biology and Mordern Biological Technology Llab Open Foundation (ZS11007).

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Authors and Affiliations

  1. Key Laboratory of Resources Biology and Biotechnology in Western China, Ministry of Education, Faculty of Life Sciences, Northwest University, Xi’an, China

    Jing Qi, Bobo Wang, Jing Li, Huanhuan Ning, Yingjuan Wang, Weina Kong & Lixin Shen

  2. Department of Oral Biology, Faculty of Dentistry, University of Manitoba, 780 Bannatyne Ave, Winnipeg, MB R3E 0W2, Canada

    Weina Kong

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  1. Jing Qi
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  2. Bobo Wang
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  3. Jing Li
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  7. Lixin Shen
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Correspondence to Lixin Shen.

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Responsible editor: Philippe Garrigues

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Qi, J., Wang, B., Li, J. et al. Genetic determinants involved in the biodegradation of naphthalene and phenanthrene in Pseudomonas aeruginosa PAO1. Environ Sci Pollut Res 22, 6743–6755 (2015). https://doi.org/10.1007/s11356-014-3833-4

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