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Alcanivorax profundimaris sp. nov., a Novel Marine Hydrocarbonoclastic Bacterium Isolated from Seawater and Deep-Sea Sediment

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Abstract

Two novel Alcanivorax-related strains, designated ST75FaO-1T and 521-1, were isolated from the seawater of the South China Sea and the deep-sea sediment of the West Pacific Ocean, respectively. Both strains are Gram-stain-negative, rod-shaped, and non-motile, and grow at 10–40 °C, pH 5.0–10.0, in the presence of 1.0–15.0% (w/v) NaCl. Their 16S rRNA gene sequences showed 99.9% similarity. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that both strains belong to the genus Alcanivorax, and share 92.9–98.1% sequence similarity with all valid type strains of this genus, with the highest similarity being to type strain Alcanivorax venustensis DSM 13974T (98.0–98.1%). Digital DNA–DNA hybridization (dDDH) and average nucleotide identity values between strains ST75FaO-1T and 521-1 were 75.7% and 97.1%, respectively, while the corresponding values with A. venustensis DSM 13974T were only 25.4–25.6% and 82.4–82.7%, respectively. The two strains contained similar major cellular fatty acids including C16:0, C18:1 ω7c/ω6c, C19:0 cyclo ω8c, C16:1 ω7c/ω6c, C12:0 3-OH, and C12:0. The genomic G + C content of strains ST75FaO-1T and 521-1 were 66.3% and 66.1%, respectively. Phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, and one unidentified polar lipid were present in both strains. On the basis of phenotypic and genotypic characteristics, the two strains represent a novel species within the genus Alcanivorax, for which the name Alcanivorax profundimaris sp. nov. is proposed. The type strain is ST75FaO-1T (= MCCC 1A17714T = KCTC 82142T).

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Acknowledgements

We thank the chief scientist Dr. Xin Liu, the whole team of the KK1904 cruise and the crew of the R/V Tan Kah Kee for collecting samples in the South China Sea.

Funding

This work was financially supported by National Key R&D Program of China (2016YFA0601202); Scientific Research Foundation of Third Institute of Oceanography, MNR (2020009); National Natural Science Foundation of China (41976107, 91851203), China Ocean Mineral Resource R&D Association program (DY135-B2-01), and National Infrastructure of Natural Resources for Science and Technology Program of China (NIMR-2020-9).

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Author notes

  1. Chunming Dong and Qiliang Lai contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, No. 184, Daxue Road, Siming District, Xiamen, 361005, Fujian, People’s Republic of China

    Chunming Dong, Qiliang Lai, Xiupian Liu, Li Gu & Zongze Shao

  2. State Key Laboratory Breeding Base of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, No. 184, Daxue Road, Siming District, Xiamen, 361005, Fujian, People’s Republic of China

    Chunming Dong, Qiliang Lai, Xiupian Liu, Li Gu & Zongze Shao

  3. Fujian Key Laboratory of Marine Genetic Resources, No. 184, Daxue Road, Siming District, Xiamen, 361005, Fujian, People’s Republic of China

    Chunming Dong, Qiliang Lai, Xiupian Liu, Li Gu & Zongze Shao

  4. State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen, 361005, Fujian, People’s Republic of China

    Yu Zhang, Zhangxian Xie & Dazhi Wang

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  1. Chunming Dong
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Contributions

ZS supervised the study. CD and QL designed the research outline. YZ collected the seawater sample from the South China Sea. CD, QL, XL and LG performed the experiments. CD drafted the manuscript. CD, QL, ZS, ZX, DW revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zongze Shao.

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Dong, C., Lai, Q., Liu, X. et al. Alcanivorax profundimaris sp. nov., a Novel Marine Hydrocarbonoclastic Bacterium Isolated from Seawater and Deep-Sea Sediment. Curr Microbiol 78, 1053–1060 (2021). https://doi.org/10.1007/s00284-020-02322-7

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