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Optimization of Growth Conditions of Acinetobacter sp. Cr1 for Removal of Heavy Metal Cr Using Central Composite Design

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Abstract

Growth conditions can significantly affect the removal efficiency of heavy metals by microorganisms. The goal of this study was enhancing the removal efficiency of Cr(VI) and improving the application of Acinetobacter sp. Cr1 (GenBank accession number of 16S rDNA sequence, MN900681). This study focused on pH, Cr(VI) concentration and culture time, which were the major influence factors for removal efficiency of Cr(VI). A central composite design was employed to optimize the removal efficiency by optimizing three variables. The optimum growth conditions were as: pH of 9.52, Cr(VI) concentration of 128.55 mg l−1, culture time of 43.30 h, and the predicted and actual maxima were 65.13% and 67.26%, respectively. Therefore, it is suggested that the strain Acinetobacter sp. Cr1 had a promising potential to be used for bioremediation of Cr(VI).

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Acknowledgements

This research was funded by National Natural Science Foundation of China (No. 31860010), Natural Science Foundation of Guangxi Zhuang autonomous region (No. 2018GXNSFAA138008), Science and Technology Program of Guangzhou, China (No. 201804010155), Scientific Research Project of Guangxi Normal University (No. 2020CX001 and 2019YR004), Foundation of Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, China (No. ERESEP2020Z04) and Ecological Doctoral Program Construction of Guangxi Normal University (No. EDPC 2018001).

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Authors and Affiliations

  1. College of Life Science, Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guangxi Normal University, No. 1, Yanzhong Road, Yanshan District, Guilin, 541006, Guangxi, China

    Bingbing Pang, Lijun Lv, Caoping Pang, Fengcai Ye & Changhua Shang

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  1. Bingbing Pang
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Contributions

BP and LL wrote the main part of the paper and performed the experiments. BP and LL contributed equally to this work. CP and FY wrote the less part of the paper. CS conceived the experiments and proofread the paper. All authors read and approved the manuscript.

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Correspondence to Changhua Shang.

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We have obtained the appropriate permission from the responsible authority for collecting heavy metals contaminated soil samples from mining areas.

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Pang, B., Lv, L., Pang, C. et al. Optimization of Growth Conditions of Acinetobacter sp. Cr1 for Removal of Heavy Metal Cr Using Central Composite Design. Curr Microbiol 78, 316–322 (2021). https://doi.org/10.1007/s00284-020-02278-8

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