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Reinekea thalattae sp. nov., a New Species of the Genus Reinekea Isolated from Surface Seawater in Sehwa Beach

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Abstract

A Gram-stain-negative, non-pigmented, curved rod-shaped, single polarly flagellated, facultatively anaerobic bacterium, designated as SSH23T, was isolated from surface seawater sample collected at the Sehwa Beach in South Korea. The novel isolate required NaCl for growth and grew optimally between 2 and 3% NaCl. Strain SSH23T showed high 16S rRNA gene sequence similarities with Reinekea marinisedimentorum DSM 15388T (96.4%), Reinekea marina KACC 17315T (96.2%), Reinekea blandensis KACC 17315T (95.9%) and Reinekea aestuarii KCTC 22813T (95.6%). The major polar lipids of strain SSH23T were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major cellular fatty acids of strain SSH23T were C16:0, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). The predominant respiratory quinone was found to be ubiquinone-8. The average nucleotide identity values of strain SSH23T with R. marinisedimentorum DSM 15388T and R. blandensis MED297T were determined to be 72.2% and 69.8%, respectively. The G+C content of the genomic DNA was 45.5 mol%. Based on genotypic, phenotypic, chemotaxonomic, and phylogenetic analyses, strain SSH23T was considered to represent a novel member of the genus Reinekea, for which the name Reinekea thalattae sp. nov. is proposed. The type strain of Reinekea thalattae is SSH23T (= KACC 21168T = NBRC 113795T).

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Acknowledgements

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002203), and by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2020R1F1A1072647).

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Authors and Affiliations

  1. Department of Life Science, Dongguk University-Seoul, Goyang, 10326, South Korea

    Inhyup Kim, Geeta Chhetri, Jiyoun Kim, Minchung Kang & Taegun Seo

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  1. Inhyup Kim
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  2. Geeta Chhetri
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  3. Jiyoun Kim
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  4. Minchung Kang
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  5. Taegun Seo
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Contributions

IK isolated the bacterium, designed the study, performed the phenotypic and biochemical characterization, wrote the original draft; GC, JK and MK helped the analysis of taxonomic data; TS designed and supervised the study, edited the original draft.

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Correspondence to Taegun Seo.

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Kim, I., Chhetri, G., Kim, J. et al. Reinekea thalattae sp. nov., a New Species of the Genus Reinekea Isolated from Surface Seawater in Sehwa Beach. Curr Microbiol 77, 4174–4179 (2020). https://doi.org/10.1007/s00284-020-02224-8

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  • DOI: https://doi.org/10.1007/s00284-020-02224-8

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