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Arenibacter lacus sp. nov., Isolated from Chilika Lagoon, India

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Abstract

A shiny dark yellow pigmented, Gram-stain-negative, rod-shaped, non-motile bacterium, designated as strain JC631T, was isolated from the sediment ecosystem of Chilika lagoon, India. Strain JC631T tolerates up to pH 10 and NaCl up to 3% (w/v). MK6 is the only respiratory quinone. Predominant (> 10%) cellular fatty acids are anteiso-C11:0, anteiso-C13:0, and anteiso-C15:0. Major polar lipids are phosphatidylethanolamine, phosphatidylcholine, a few unidentified phospholipids, and a few unidentified aminolipids. 16S rRNA gene sequencing showed that strain JC631T shared the highest sequence identity (98.7%) with Arenibacter latericius KMM 426T followed by Arenibacter certesii CCUG 48006T (98.6%) and other members of the genus Arenibacter (< 97%). The genome size of strain JC631T is 4.16 Mb with a GC content of 40.8 mol%. Strain JC631T has ANI scores of 78.3% and 78.1%; dDDH values of 22.2% and 21.8%, respectively, with the type strains of A. latericius and A. certesii. The genomic distinction is well supported by chemotaxonomic characteristics and differential physiological properties. This supports strain JC631T to be described as a new species of the genus Arenibacter, named as Arenibacter lacus sp. nov. The type strain is JC631T (= KCTC 72002T = NBRC 114071T).

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Acknowledgements

DK and GK thank the UGC and DBT, New Delhi, for the award of Dr. D.S. Kothari post-doctoral fellowship and SRF fellowship, respectively. JU thanks TEQIP III for research fellowship. ChVR thank the Department of Biotechnology, Government of India, for the award of Tata Innovation fellowship. Department of Science and Technology and University Grants Commission are acknowledged for providing infrastructural facilities as FIST and SAP (DRS). Infrastructure facility created through DST-FIST (UoH and JNTUH) and TEQIP (for JNTUH) are acknowledged. Authors thank Belgian Coordinated Collections of Microorganisms (BCCM) and Korean Collection for Type Cultures (KCTC) for providing A. latericius LMG 19693T and A. certesii KCTC 12113T, respectively, as gratis, in exchange.

Funding

This work received no specific grant from any funding agency. Financial support received from Council for scientific and industrial research (CSIR), New Delhi, is acknowledged.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046, India

    Dhanesh Kumar, Nandardhane Smita, Gaurav Kumar, Gandham Suresh & Chintalapati Venkata Ramana

  2. Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University Hyderabad, Kukatpally, Hyderabad, 500085, India

    Uppada Jagadeeshwari & Chintalapati Sasikala

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  1. Dhanesh Kumar
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequences of the strains JC631T is LR215975. The GenBank/EMBL/DDBJ accession number for the whole-genome shotgun sequence is VVIN00000000.

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Kumar, D., Smita, N., Kumar, G. et al. Arenibacter lacus sp. nov., Isolated from Chilika Lagoon, India. Curr Microbiol 77, 4152–4159 (2020). https://doi.org/10.1007/s00284-020-02205-x

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