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Characterization of Extracellular Biosurfactants Expressed by a Pseudomonas putida Strain Isolated from the Interior of Healthy Roots from Sida hermaphrodita Grown in a Heavy Metal Contaminated Soil

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Abstract

Pseudomonas putida E41 isolated from root interior of Sida hermaphrodita (grown on a field contaminated with heavy metals) showed high biosurfactant activity. In this paper, we describe data from mass spectrometry and genome analysis, to improve our understanding on the phenotypic properties of the strain. Supernatant derived from P. putida E41 liquid culture exhibited a strong decrease in the surface tension accompanied by the ability for emulsion stabilization. We identified extracellular lipopeptides, putisolvin I and II expression but did not detect rhamnolipids. Their presence was confirmed by matrix-assisted laser desorption and ionization (MALDI) TOF/TOF technique. Moreover, ten phospholipids (mainly phosphatidylethanolamines PE 33:1 and PE 32:1) which were excreted by vesicles were also detected. In contrast the bacterial cell pellet was dominated by phosphatidylglycerols (PGs), which were almost absent in the supernatant. It seems that the composition of extracellular (secreted to the environment) and cellular lipids in this strain differs. Long-read sequencing and complete genome reconstruction allowed the identification of a complete putisolvin biosynthesis pathway. In the genome of P. putida E41 were also found all genes involved in glycerophospholipid biosynthesis, and they are likely responsible for the production of detected phospholipids. Overall this is the first report describing the expression of extracellular lipopeptides (identified as putisolvins) and phospholipids by a P. putida strain, which might be explained by the need to adapt to the highly contaminated environment.

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Acknowledgements

This work was carried out under Maria Curie-Sklodowska Actions IAPP of 7th UE Programme for Research, Technological Development and Demonstration under Grant Agreement No 610797, acronym “Phyto2Energy”.

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Authors and Affiliations

  1. Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Łódź, Banacha 12/16 street, 90-237, Łódź, Poland

    Przemysław Bernat & Katarzyna Paraszkiewicz

  2. Comparative Microbiome Analysis, Helmholtz Zentrum München, 85764, Neuherberg, Germany

    Joseph Nesme & Michael Schloter

  3. Section of Microbiology, Department of Biology, Faculty of Science, University of Copenhagen, 2100, Copenhagen, Denmark

    Joseph Nesme

  4. Institute for Ecology of Industrial Areas, Unit of Environmental Microbiology, Kossutha 6, 40-844, Katowice, Poland

    Grażyna Płaza

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  1. Przemysław Bernat
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Bernat, P., Nesme, J., Paraszkiewicz, K. et al. Characterization of Extracellular Biosurfactants Expressed by a Pseudomonas putida Strain Isolated from the Interior of Healthy Roots from Sida hermaphrodita Grown in a Heavy Metal Contaminated Soil. Curr Microbiol 76, 1320–1329 (2019). https://doi.org/10.1007/s00284-019-01757-x

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