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Streptococcus periodonticum sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion

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Abstract

A novel facultative anaerobic and Gram-stain-positive coccus, designated strain ChDC F135T, was isolated from human subgingival dental plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene (16S rDNA) sequence of strain ChDC F135T was closest to that of Streptococcus sinensis HKU4T (98.2%), followed by Streptococcus intermedia SK54T (97.0%), Streptococcus constellatus NCTC11325T (96.0%), and Streptococcus anginosus NCTC 10713T (95.7%). In contrast, phylogenetic analysis based on the superoxide dismutase gene (sodA) and the RNA polymerase beta-subunit gene (rpoB) showed that the nucleotide sequence similarities of strain ChDC F135T were highly similar to the corresponding genes of S. anginosus NCTC 10713T (99.2% and 97.6%, respectively), S. constellatus NCTC11325T (87.8% and 91.4%, respectively), and S. intermedia SK54T (85.8% and 91.2%, respectively) rather than those of S. sinensis HKU4T (80.5% and 82.6%). The complete genome of strain ChDC F135T consisted of 1,901,251 bp and the G+C content was 38.9 mol %. Average nucleotide identity value between strain ChDC F135T and S. sinensis HKU4T or S. anginosus NCTC 10713T were 75.7% and 95.6%, respectively. The C14:0 composition of the cellular fatty acids of strain ChDC F135T (32.8%) was different from that of S. intermedia (6–8%), S. constellatus (6–13%), and S. anginosus (13–20%). Based on the results of phylogenetic and phenotypic analysis, strain ChDC F135T (= KCOM 2412T = JCM 33300T) was classified as a type strain of a novel species of the genus Streptococcus, for which we proposed the name Streptococcus periodonticum sp. nov.

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Acknowledgments

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science and ICT (Grant No. 2017M3A9B8065844) and in part by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (Grant No. 2018R1A2B5002239).

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  1. Yun Kyong Lim and Soon-Nang Park have contributed equally to this work.

Authors and Affiliations

  1. Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Republic of Korea

    Yun Kyong Lim, Soon-Nang Park & Joong-Ki Kook

  2. Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea

    Jeong Hwan Shin

  3. ABS Research Support Center, KRIBB, Daejeon, Republic of Korea

    Young-Hyo Chang, Yeseul Shin & Jayoung Paek

  4. Vitabio, Inc, Daejeon, Republic of Korea

    Hongik Kim

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DPD number: TA00863. GenBank accession number of 16S rRNA gene for strain ChDC F135 T: MK452275. GenBank accession number of genome for strain ChDC F135T: CP034543.

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284_2019_1695_MOESM1_ESM.pptx

Supplementary material 1—Supplementary Fig S1. Maximum likelihood (A) and the minimum evolution (B) phylogenetic tree based on 16S rDNA sequences of strain ChDC F135T and type strains of related species. Stability of phylogenetic tree was assessed by bootstrap analysis of 1,000 replicates with MEGA version 6.06 [27]. Bars indicate 0.01 (A) or 0.005 (B) changes per nucleotide position (PPTX 90 kb)

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Lim, Y.K., Park, SN., Shin, J.H. et al. Streptococcus periodonticum sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion. Curr Microbiol 76, 835–841 (2019). https://doi.org/10.1007/s00284-019-01695-8

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